N-domain angiotensin I-converting enzyme with 80 kDa as a possible genetic marker of hypertension

N-domain angiotensin I-converting enzyme with 80 kDa as a possible genetic marker of hypertension

Author Marques, Georgia Daniela Marcusso Autor UNIFESP Google Scholar
Quinto, Beata Marie Redublo Autor UNIFESP Google Scholar
Plavnik, Frida Liane Autor UNIFESP Google Scholar
Krieger, J. E. Google Scholar
Marson, Odair Autor UNIFESP Google Scholar
Casarini, Dulce Elena Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract We have previously described angiotensin I-converting enzyme (ACE) forms in urine of normotensive (190 and 65 kDa) and hypertensive patients (90 and 65 kDa, N-domain ACEs). Based on the results described above, experimental and genetic models of hypertension were investigated to distinguish hemodynamic and genetic influence on the generation of ACE profile in urine: Wistar-Kyoto and Brown Norway rats (WKY and BN), spontaneously and stroke-prone spontaneously hypertensive rats (SHR and SHR-SP), one kidney/one clip rats (1K1C), deoxycorticosterone acetate (DOCA) salt-treated and untreated rats, and enalapril-treated SHR (SHRen). Two peaks with ACE activity were separated from the urine of WKY and BN rats submitted to an AcA-44 column, WK-1/BN-1 (190 kDa), and WK-2/BN-2 (65 kDa), as described for urine of normotensive subjects. the same results were obtained for urine of 1K1C and DOCA salt-treated and untreated rats, analyzed to evaluate the influence of hemodynamic factors in the ACE profile in urine. the urine from SHR, SHR-SP, and SHRen presented 80 (S-1, SP-1, Sen-1) and 65 (S-2, SP-2, Sen-2) kDa ACE forms, differing from the urine profile of normotensive rats, but similar to that described for hypertensive patients. the presence of 80 kDa ACE in urine of SHR, SHR-SP, and SHRen and its absence in urine of experimental hypertensive rats (1K1C and DOCA salt) support the hypothesis that this enzyme could be a possible genetic marker of hypertension. Taken together, our results provide evidence that ACE forms with 90/80 kDa isolated from the urine of hypertensive subjects and genetic hypertensive animals behaves as a possible genetic marker of hypertension and not as a marker of high blood pressure.
Keywords angiotensin-converting enzyme
hypertension, genetic
Language English
Date 2003-10-01
Published in Hypertension. Philadelphia: Lippincott Williams & Wilkins, v. 42, n. 4, p. 693-701, 2003.
ISSN 0194-911X (Sherpa/Romeo, impact factor)
Publisher Lippincott Williams & Wilkins
Extent 693-701
Origin http://dx.doi.org/10.1161/01.HYP.0000085784.18572.CB
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000185679000171
URI http://repositorio.unifesp.br/handle/11600/27421

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