Helicobacter pylori and cagA and vacA gene status in children from Brazil with chronic gastritis

Helicobacter pylori and cagA and vacA gene status in children from Brazil with chronic gastritis

Author Gatti, L. L. Google Scholar
Agostinho, F. Google Scholar
Labio, R. de Google Scholar
Piason, F. B. Google Scholar
Silva, L. C. da Google Scholar
Queiroz, V. F. de Google Scholar
Peres, C. A. Google Scholar
Barbieri, D. Google Scholar
Smith, MDC Google Scholar
Payao, SLM Google Scholar
Institution Fac Med Marilia
Universidade Federal de São Paulo (UNIFESP)
FAMEMA
Universidade de São Paulo (USP)
Univ Sagrado
Abstract Helicobacter pylori has been shown to be strongly associated with chronic gastritis, gastric and duodenal ulceration, and is a risk factor for gastric carcinoma. Histology, urease, culture, and polymerase chain reaction have been employed as for H. pylori diagnostic methods, pre and post treatment or during follow-up of dyspeptic adult individuals referred for endoscopy. in order to obtain a more-sensitive and specific method for H. pylori detection, we evaluated gastric body and antrum biopsies of 134 consecutive Brazilian consecutive dyspeptic children aged 1-16 years by rapid urease test, histology and polymerase chain reaction using two pairs of oligonucleotides. Our results indicated that polymerase chain reaction with Southern blotting and hybridization with specific chemiluminescent probes increased the number of positive H. pylori patients by 35%. the genotyping of H. pylori strains directly from gastric biopsy using the same nucleic acid methodology revealed that there is no association of chronic gastritis in our infant patients with vacA s1 and the presence of the cagA gene. These data suggest an initial infection of children with normal mucosa and probably others factors than vacA s1 genotype or the presence of the cagA gene are associated with the onset of gastric disease. Altogether, our results reinforce the need for using more sensitive diagnostic methods in order to understand the role of H. pylori in the genesis of gastric disease in children and its progression in adults.
Keywords Helicobacter pylori
polymerase chain reaction
biopsy-based diagnosis
cag A
vacA alleles
chronic gastritis
dyspeptic children
Language English
Date 2003-09-01
Published in Clinical and Experimental Medicine. New York: Springer-verlag, v. 3, n. 3, p. 166-172, 2003.
ISSN 1591-8890 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 166-172
Origin http://dx.doi.org/10.1007/s10238-003-0021-0
Access rights Closed access
Type Article
Web of Science ID WOS:000186822500006
URI http://repositorio.unifesp.br/handle/11600/27386

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