Cloning and characterisation of a cysteine proteinase gene expressed in amastigotes of Leishmania (L.) amazonensis

Cloning and characterisation of a cysteine proteinase gene expressed in amastigotes of Leishmania (L.) amazonensis

Author Lasakosvitsch, F. Google Scholar
Gentil, L. G. Google Scholar
Santos, MRM dos Google Scholar
Silveira, J. F. da Google Scholar
Barbieri, C. L. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract The present study describes the cloning and characterisation of a gene encoding a cysteine proteinase isoform, Llacys1, expressed in amastigote forms of Leishmania (L.) amazonensis. Recombinant clones containing the Llacys1 gene were isolated from genomic DNA by PCR amplification and screening of an amastigote cDNA library. Sequence analysis of the Llacys1 gene showed a high identity to sequence of Leishmania (L.) pifanoi Lpcys1, Leishmania (L.) major cpa, Leishmania (L.) mexicana LCPa, and Leishmania (L.) chagasi Ldccys2. the Llacys1 gene is present in a single copy per L. (L.) amazonensis haploid genome and was mapped on a chromosome of approximately 700 kb. Two transcripts of the Llacys1 gene were identified, one of 2.4 kb transcribed in both forms of L (L.) amazonensis, and another of 1.6 kb weakly expressed in amastigotes. Related forms of Llacys1 gene exist in other species of Leishmania genus, including L (L.) major, L. (L.) mexicana, L. (L.) chagasi and Leishmania (V.) braziliensis. the Llacys1 expression in Escherichia coli was obtained when the nucleotide sequence corresponding to the signal sequence was deleted, suggesting that this signal sequence was recognised by Escherichia coli and cleaved, generating a truncated protein. (C) 2003 Australian Society for Parasitology Inc. Published by Elsevier B.V. All rights reserved
Keywords Leishmania (L.) amazonensis
amastigotes
cysteine proteinase isoforms
gene expression
Language English
Date 2003-04-01
Published in International Journal for Parasitology. Oxford: Pergamon-Elsevier B.V., v. 33, n. 4, p. 445-454, 2003.
ISSN 0020-7519 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 445-454
Origin http://dx.doi.org/10.1016/S0020-7519(03)00010-9
Access rights Closed access
Type Article
Web of Science ID WOS:000182644100013
URI http://repositorio.unifesp.br/handle/11600/27186

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