Heparan sulfate proteoglycans mediate the invasion of cardiomyocytes by Trypanosoma cruzi

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dc.contributor.author Calvet, C. M.
dc.contributor.author Toma, L.
dc.contributor.author De Souza, F. R.
dc.contributor.author De Meirelles, M. D.
dc.contributor.author Pereira, MCS
dc.date.accessioned 2016-01-24T12:33:45Z
dc.date.available 2016-01-24T12:33:45Z
dc.date.issued 2003-03-01
dc.identifier http://dx.doi.org/10.1111/j.1550-7408.2003.tb00240.x
dc.identifier.citation Journal of Eukaryotic Microbiology. Lawrence: Soc Protozoologists, v. 50, n. 2, p. 97-103, 2003.
dc.identifier.issn 1066-5234
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/27172
dc.description.abstract Cytoadherence is an important step for the invasion of a mammalian host cell by Trypanosoma cruzi. Cell surface macromolecules are implicated in the T. cruzi-cardiomyocyte recognition process. Therefore, we investigated the role of cell surface proteoglycans during this invasion process and analyzed their expression after the parasite infected the target cells. Treatment of trypomastigote forms of T. cruzi with soluble heparan sulfate resulted in a significant inhibition in successful invasion, while chondroitin sulfate had no effect. Removal of sulfated glycoconjugates from the cardiomyocyte surface using glycosaminoglycan (GAG) lyases demonstrated the specific binding of the parasites to heparan sulfate proteoglycans. Infection levels were reduced by 42% when the host cells were previously treated with heparitinase II. No changes were detected in the expression of GAGS infected cardiomyocytes even after 96 h of infection. Our data demonstrate that heparan sulfate proteoglycans, but not chondroitin sulfate, mediate both attachment and invasion of cardiomyocytes by T. cruzi. en
dc.format.extent 97-103
dc.language.iso eng
dc.publisher Soc Protozoologists
dc.relation.ispartof Journal of Eukaryotic Microbiology
dc.rights Acesso restrito
dc.subject cardiomyocytes en
dc.subject glycosaminoglycans en
dc.subject proteoglycans en
dc.subject Trypanosoma cruzi en
dc.title Heparan sulfate proteoglycans mediate the invasion of cardiomyocytes by Trypanosoma cruzi en
dc.type Artigo
dc.contributor.institution Inst Oswaldo Cruz
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Inst Oswaldo Cruz, Dept Ultraestrutura & Biol Celular, Fdn Oswald Cruz, BR-21045900 Rio de Janeiro, Brazil
dc.description.affiliation UNIFESP, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifesp UNIFESP, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.identifier.doi 10.1111/j.1550-7408.2003.tb00240.x
dc.description.source Web of Science
dc.identifier.wos WOS:000182532900004


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