Heparan sulfate proteoglycans mediate the invasion of cardiomyocytes by Trypanosoma cruzi

Heparan sulfate proteoglycans mediate the invasion of cardiomyocytes by Trypanosoma cruzi

Author Calvet, C. M. Google Scholar
Toma, L. Google Scholar
De Souza, F. R. Google Scholar
De Meirelles, M. D. Google Scholar
Pereira, MCS Google Scholar
Institution Inst Oswaldo Cruz
Universidade Federal de São Paulo (UNIFESP)
Abstract Cytoadherence is an important step for the invasion of a mammalian host cell by Trypanosoma cruzi. Cell surface macromolecules are implicated in the T. cruzi-cardiomyocyte recognition process. Therefore, we investigated the role of cell surface proteoglycans during this invasion process and analyzed their expression after the parasite infected the target cells. Treatment of trypomastigote forms of T. cruzi with soluble heparan sulfate resulted in a significant inhibition in successful invasion, while chondroitin sulfate had no effect. Removal of sulfated glycoconjugates from the cardiomyocyte surface using glycosaminoglycan (GAG) lyases demonstrated the specific binding of the parasites to heparan sulfate proteoglycans. Infection levels were reduced by 42% when the host cells were previously treated with heparitinase II. No changes were detected in the expression of GAGS infected cardiomyocytes even after 96 h of infection. Our data demonstrate that heparan sulfate proteoglycans, but not chondroitin sulfate, mediate both attachment and invasion of cardiomyocytes by T. cruzi.
Keywords cardiomyocytes
glycosaminoglycans
proteoglycans
Trypanosoma cruzi
Language English
Date 2003-03-01
Published in Journal of Eukaryotic Microbiology. Lawrence: Soc Protozoologists, v. 50, n. 2, p. 97-103, 2003.
ISSN 1066-5234 (Sherpa/Romeo, impact factor)
Publisher Soc Protozoologists
Extent 97-103
Origin http://dx.doi.org/10.1111/j.1550-7408.2003.tb00240.x
Access rights Closed access
Type Article
Web of Science ID WOS:000182532900004
URI http://repositorio.unifesp.br/handle/11600/27172

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