Effect of bradykinin and PMA on the synthesis of proteoglycans during the cell cycle of endothelial cells in culture

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dc.contributor.author Moreira, C. R.
dc.contributor.author Porcionatto, M. A.
dc.contributor.author Dietrich, C. P.
dc.contributor.author Nader, H. B.
dc.date.accessioned 2016-01-24T12:33:45Z
dc.date.available 2016-01-24T12:33:45Z
dc.date.issued 2003-03-01
dc.identifier http://dx.doi.org/10.1016/S1567-5769(02)00262-X
dc.identifier.citation International Immunopharmacology. Amsterdam: Elsevier B.V., v. 3, n. 3, p. 293-298, 2003.
dc.identifier.issn 1567-5769
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/27168
dc.description.abstract Bradykinin (BK) and phorbol 12-myristate-13-acetate (PMA) were used in the present work to study the biosynthesis of proteoglycans (PG) during the cell cycle of endothelial cells. PMA, an activator of PKC, stimulated the synthesis of heparan sulfate proteoglycan (HSPG) secreted to the medium of endothelial cells mainly during the G, phase of the cell cycle [J. Cell. Biochem. 70 (1998) 563]. BK is a vasoactive peptide that increases calcium levels inside the cells indirectly stimulating PKC. Treatment of the endothelial cells with BK, as well as PMA, stimulated the synthesis of HSPG secreted to the medium and produced an antimitogenic effect on the cell cycle. These results led to the conclusion that PKC is directly involved in the synthesis of HSPG secreted to the medium. Also, comparing the effect showed by BK with PMA, one may suggest that different PKC isoforms are involved in these two processes and that their isoforms are mainly Ca2+ dependent. (C) 2002 Elsevier Science B.V. All rights reserved. en
dc.format.extent 293-298
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof International Immunopharmacology
dc.rights Acesso restrito
dc.subject heparan sulfate and growth factors en
dc.subject heparan sulfate and protein kinase C en
dc.subject cell cycle and proteoglycan en
dc.subject inhibition of DNA synthesis and protein kinase C en
dc.subject bradykinin and heparan sulfate synthesis en
dc.title Effect of bradykinin and PMA on the synthesis of proteoglycans during the cell cycle of endothelial cells in culture en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Universidade Federal de São Paulo, Dept Bioquim Biol Mol, Escola Paulista Med, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Bioquim Biol Mol, Escola Paulista Med, BR-04044020 São Paulo, Brazil
dc.identifier.doi 10.1016/S1567-5769(02)00262-X
dc.description.source Web of Science
dc.identifier.wos WOS:000182034900002



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