Effect of bradykinin and PMA on the synthesis of proteoglycans during the cell cycle of endothelial cells in culture

Effect of bradykinin and PMA on the synthesis of proteoglycans during the cell cycle of endothelial cells in culture

Author Moreira, C. R. Google Scholar
Porcionatto, M. A. Google Scholar
Dietrich, C. P. Google Scholar
Nader, H. B. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Bradykinin (BK) and phorbol 12-myristate-13-acetate (PMA) were used in the present work to study the biosynthesis of proteoglycans (PG) during the cell cycle of endothelial cells. PMA, an activator of PKC, stimulated the synthesis of heparan sulfate proteoglycan (HSPG) secreted to the medium of endothelial cells mainly during the G, phase of the cell cycle [J. Cell. Biochem. 70 (1998) 563]. BK is a vasoactive peptide that increases calcium levels inside the cells indirectly stimulating PKC. Treatment of the endothelial cells with BK, as well as PMA, stimulated the synthesis of HSPG secreted to the medium and produced an antimitogenic effect on the cell cycle. These results led to the conclusion that PKC is directly involved in the synthesis of HSPG secreted to the medium. Also, comparing the effect showed by BK with PMA, one may suggest that different PKC isoforms are involved in these two processes and that their isoforms are mainly Ca2+ dependent. (C) 2002 Elsevier Science B.V. All rights reserved.
Keywords heparan sulfate and growth factors
heparan sulfate and protein kinase C
cell cycle and proteoglycan
inhibition of DNA synthesis and protein kinase C
bradykinin and heparan sulfate synthesis
Language English
Date 2003-03-01
Published in International Immunopharmacology. Amsterdam: Elsevier B.V., v. 3, n. 3, p. 293-298, 2003.
ISSN 1567-5769 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 293-298
Origin http://dx.doi.org/10.1016/S1567-5769(02)00262-X
Access rights Closed access
Type Article
Web of Science ID WOS:000182034900002
URI http://repositorio.unifesp.br/handle/11600/27168

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