Human cutaneous melanoma expresses a significant phosphate-dependent glutaminase activity: A comparison with the surrounding skin of the same patient

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dc.contributor.author Zacharias, DPM
dc.contributor.author Lima, MMR
dc.contributor.author Souza, A. L.
dc.contributor.author Santos, IDDO
dc.contributor.author Enokiara, M.
dc.contributor.author Michalany, N.
dc.contributor.author Curi, R.
dc.date.accessioned 2016-01-24T12:33:44Z
dc.date.available 2016-01-24T12:33:44Z
dc.date.issued 2003-03-01
dc.identifier http://dx.doi.org/10.1002/cbf.997
dc.identifier.citation Cell Biochemistry and Function. W Sussex: John Wiley & Sons Ltd, v. 21, n. 1, p. 81-84, 2003.
dc.identifier.issn 0263-6484
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/27161
dc.description.abstract The protein content and the activity and type of phosphate-dependent glutaminase were determined in freshly pigmented lesions obtained from human melanoma and adjacent skin. Significant phosphate-dependent glutaminase activity was found in both the melanoma and non-pigmented adjacent skin areas. A comparison between the pigmented and adjacent skin areas suggests the occurrence of gradual metabolic changes that result in an increased protein content in the centre of the neoplasia. the presence of a kidney-type glutaminase (K-m of 2-5 mM) indicates a high sensitivity of the melanoma to variations in glutamine plasma levels (0.6 to 1 mM). These data lead us to postulate that glutamine supply is an important factor for melanoma cell proliferation, being a source of nitrogen for DNA and RNA synthesis. the intense neovascularization observed in melanoma ensures the oxygen supply that is required for glutamine oxidation. These findings support the proposition that glutamine is an important fuel for melanoma. Copyright (C) 2002 John Wiley Sons, Ltd. en
dc.format.extent 81-84
dc.language.iso eng
dc.publisher Wiley-Blackwell
dc.relation.ispartof Cell Biochemistry and Function
dc.rights Acesso restrito
dc.title Human cutaneous melanoma expresses a significant phosphate-dependent glutaminase activity: A comparison with the surrounding skin of the same patient en
dc.type Artigo
dc.rights.license http://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ São Paulo, ICB, Dept Fisiol & Biofis, Lab Fisiol Celular, BR-05508900 São Paulo, Brazil
dc.description.affiliation UNIFESP, São Paulo, Brazil
dc.description.affiliationUnifesp UNIFESP, São Paulo, Brazil
dc.identifier.doi 10.1002/cbf.997
dc.description.source Web of Science
dc.identifier.wos WOS:000181097100012



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