Human cutaneous melanoma expresses a significant phosphate-dependent glutaminase activity: A comparison with the surrounding skin of the same patient

Human cutaneous melanoma expresses a significant phosphate-dependent glutaminase activity: A comparison with the surrounding skin of the same patient

Author Zacharias, DPM Google Scholar
Lima, MMR Google Scholar
Souza, A. L. Google Scholar
Santos, IDDO Google Scholar
Enokiara, M. Google Scholar
Michalany, N. Google Scholar
Curi, R. Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract The protein content and the activity and type of phosphate-dependent glutaminase were determined in freshly pigmented lesions obtained from human melanoma and adjacent skin. Significant phosphate-dependent glutaminase activity was found in both the melanoma and non-pigmented adjacent skin areas. A comparison between the pigmented and adjacent skin areas suggests the occurrence of gradual metabolic changes that result in an increased protein content in the centre of the neoplasia. the presence of a kidney-type glutaminase (K-m of 2-5 mM) indicates a high sensitivity of the melanoma to variations in glutamine plasma levels (0.6 to 1 mM). These data lead us to postulate that glutamine supply is an important factor for melanoma cell proliferation, being a source of nitrogen for DNA and RNA synthesis. the intense neovascularization observed in melanoma ensures the oxygen supply that is required for glutamine oxidation. These findings support the proposition that glutamine is an important fuel for melanoma. Copyright (C) 2002 John Wiley Sons, Ltd.
Language English
Date 2003-03-01
Published in Cell Biochemistry and Function. W Sussex: John Wiley & Sons Ltd, v. 21, n. 1, p. 81-84, 2003.
ISSN 0263-6484 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 81-84
Origin http://dx.doi.org/10.1002/cbf.997
Access rights Closed access
Type Article
Web of Science ID WOS:000181097100012
URI http://repositorio.unifesp.br/handle/11600/27161

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