Identification of Mycobacterium avium genotypes with distinctive traits by combination of IS1245-based restriction fragment length polymorphism and restriction analysis of hsp65

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dc.contributor.author Oliveira, R. S. [UNIFESP]
dc.contributor.author Sircili, M. P. [UNIFESP]
dc.contributor.author Oliveira, EMD
dc.contributor.author Balian, S. C.
dc.contributor.author Ferreira-Neto, J. S.
dc.contributor.author Leao, S. C. [UNIFESP]
dc.date.accessioned 2016-01-24T12:33:38Z
dc.date.available 2016-01-24T12:33:38Z
dc.date.issued 2003-01-01
dc.identifier http://dx.doi.org/10.1128/JCM.41.1.44-49.2003
dc.identifier.citation Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 41, n. 1, p. 44-49, 2003.
dc.identifier.issn 0095-1137
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/27081
dc.description.abstract One-hundred eight Mycobacterium avium isolates from pigs, humans, birds, and bovines were typed by the IS1245-based restriction fragment length polymorphism (RFLP) method and PCR-restriction enzyme analysis (PRA) of hsp65. Nine clusters of isolates showing more than 80% similarity in their RFLP profiles were detected. the largest cluster (cluster B) included 32 of 79 pig isolates (40.5%), 3 of 25 human isolates (12%), and 1 of 2 bovine isolates, comprising 33% of all isolates. the second largest cluster (cluster A) included 18 pig isolates (22.8%) and 6 human isolates (24%). Six smaller clusters included six pig isolates (clusters C and D), four and two human isolates (clusters E and F, respectively), two pig isolates (cluster 1), and two pig isolates plus one bovine isolate and the avian purified protein derivative strain (cluster H). Cluster G represented the bird-type profile and included the bird isolate in this series, one pig isolate, plus reference strain R13. PRA revealed four allelic variants. Seventy-seven isolates were identified as M. avium PRA variant 1, 24 were identified as M. avium PRA variant 11, 6 were identified as M. avium PRA variant 111, and 1 was identified as M. avium PRA variant IV. Except for three isolates from cluster B, each of the RFLP clusters was associated with a single PRA pattern. Isolates with unique (nonclustered) RFLP profiles were distributed between PRA variants I and II, and there was one unique isolate of PRA variant IV. These observations are consistent with divergent evolution within M. avium, resulting in the emergence of distinct lineages with particular competence to infect animals and humans. en
dc.format.extent 44-49
dc.language.iso eng
dc.publisher Amer Soc Microbiology
dc.relation.ispartof Journal of Clinical Microbiology
dc.rights Acesso aberto
dc.title Identification of Mycobacterium avium genotypes with distinctive traits by combination of IS1245-based restriction fragment length polymorphism and restriction analysis of hsp65 en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Universidade de São Paulo (USP)
dc.description.affiliation Universidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliation USP, FMVZ, Dept Med Vet Preventiva & Saude Anim, BR-09500900 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.identifier.file WOS000180406700006.pdf
dc.identifier.doi 10.1128/JCM.41.1.44-49.2003
dc.description.source Web of Science
dc.identifier.wos WOS:000180406700006



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