Molecular identification of Paracoccidioides brasiliensis by 5 ' nuclease assay

Molecular identification of Paracoccidioides brasiliensis by 5 ' nuclease assay

Author Semighini, C. P. Google Scholar
Camargo, Z. P. de Google Scholar
Puccia, R. Google Scholar
Goldman, MHS Google Scholar
Goldman, G. H. Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract A PCR assay based on the 5' nuclease assay using a fluorescent probe derived from the sequence of the gene coding for the 43,000-Da (gp43) antigen was developed to detect Paracoccidioides brasiliensis. the assay could detect at least 10 copies of this DNA sequence, providing efficient accuracy to be useful for diagnosis of paracoccidioidomycosis. (C) 2002 Elsevier Science Inc. All rights reserved.
Language English
Date 2002-12-01
Published in Diagnostic Microbiology and Infectious Disease. New York: Elsevier B.V., v. 44, n. 4, p. 383-386, 2002.
ISSN 0732-8893 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 383-386
Origin http://dx.doi.org/10.1016/S0732-8893(02)00472-8
Access rights Closed access
Type Article
Web of Science ID WOS:000180649300011
URI http://repositorio.unifesp.br/handle/11600/27063

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