Role of beta-D-galactofuranose in Leishmania major macrophage invasion

Role of beta-D-galactofuranose in Leishmania major macrophage invasion

Autor Suzuki, Erika Autor UNIFESP Google Scholar
Tanaka, America K. Autor UNIFESP Google Scholar
Toledo, Marcos S. Autor UNIFESP Google Scholar
Takahashi, Helio K. Autor UNIFESP Google Scholar
Straus, Anita H. Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo The role of glycosylinositol phospholipid 1 (GIPL-1) of Leishmania (Leishmania) major in the interaction of promastigotes and amastigotes with macrophages was analyzed. Monoclonal antibody MEST-1, which recognizes glycolipids containing terminal galactofuranose (Galf) residues (E. Suzuki, A S. Toledo, H. K. Takahashi, and A. H. Straus, Glycobiology 7:463-468, 1997), was used to detect GIPL-1 in Leishmania by indirect immunofluorescence and to analyze its role in macrophage infectivity. L. major promastigotes showed intense fluorescence with MEST-1, and GIPL-1 was detected in both amastigote and promastigote forms by high-performance thin-layer chromatography immunostaining by using MEST-1. Delipidation of L. major promastigotes with isopropanol-hexane-water eliminated the MEST-1 reactivity, confirming that only GIPL-1 is recognized in either amastigotes or promastigotes of this species. the biological role of GIPL-1 in the ability of L. major to invade macrophages was studied by using either Fab fragments of MEST-1 or methylglycosides. Preincubation of parasites with Fab fragments reduced macrophage infectivity in about 80% of the promastigotes and 30% of the amastigotes. Preincubation of peritoneal macrophages with p-nitrophenyl-beta-galacto-furanoside (10 mM) led to significant (similar to80%) inhibition of promastigote infectivity. These data suggest that a putative new receptor recognizing beta-D-Galf is associated with L. major macrophage infectivity and that GIPL-1 containing a terminal Galf residue is involved in the L. major-macrophage interaction.
Idioma Inglês
Data 2002-12-01
Publicado em Infection and Immunity. Washington: Amer Soc Microbiology, v. 70, n. 12, p. 6592-6596, 2002.
ISSN 0019-9567 (Sherpa/Romeo, fator de impacto)
Editor Amer Soc Microbiology
Extensão 6592-6596
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000179377600012

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