Endogenous interleukin-4 downregulates the type 1 CD4 T cell-mediated immune response induced by intramuscular DNA immunization

Endogenous interleukin-4 downregulates the type 1 CD4 T cell-mediated immune response induced by intramuscular DNA immunization

Autor Rodrigues, M. M. Google Scholar
Soares, MBP Google Scholar
Vasconcelos, JRC Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Fdn Oswaldo Cruz
Resumo Intramuscular (i.m.) administration of eukaryotic plasmid vectors containing foreign genes is a general immunization strategy capable of inducing protective type 1 immune responses against viral, bacterial, fungal, and parasitic infections. We have described that immunization with a plasmid containing a gene encoding a parasite antigen elicits specific type 1 protective immune responses against experimental infection with the human protozoan parasite Trypanosoma cruzi. However, we had evidence suggesting that DNA immunization concomitantly activated specific type 2 immune responses. To determine precisely the influence of the type 2 cytokine interleukin-4 (IL-4) during DNA immunization, we compared the immune responses of genetically modified IL-4-deficient or wild-type (wt) BALB/c mice. IL-4-deficient mice had a significantly lower ratio of specific serum IgG1/IgG2a, and on in vitro restimulation with antigen, their spleen cells secreted significantly higher amounts of interferon-gamma (IFN-gamma). in contrast, absence of IL-4 did not affect total serum antibody response, T cell proliferative responses, or activation of IFN-gamma-producing CD8(+) T cells. Our results suggested that in contrast to conventional adjuvants, such as alum and complete Freund's adjuvant, specific IgG1 in DNA-immunized BALB/c mice was highly dependent on IL-4. To our knowledge, our study provides the first evidence that endogenous IL-4 selectively downregulates the type 1 CD4(+) T cell-mediated immune response induced by i.m. genetic immunization, a fact that may have implications for the design of certain DNA vaccines.
Idioma Inglês
Data de publicação 2002-11-01
Publicado em Journal of Interferon and Cytokine Research. Larchmont: Mary Ann Liebert Inc Publ, v. 22, n. 11, p. 1137-1141, 2002.
ISSN 1079-9907 (Sherpa/Romeo, fator de impacto)
Publicador Mary Ann Liebert Inc Publ
Extensão 1137-1141
Fonte http://dx.doi.org/10.1089/10799900260442566
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000179813000008
Endereço permanente http://repositorio.unifesp.br/handle/11600/27035

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