Changes of sialomolecules during the dimethylsulfoxide-induced differentiation of Herpetomonas samuelpessoai

Changes of sialomolecules during the dimethylsulfoxide-induced differentiation of Herpetomonas samuelpessoai

Author Santos, ALS dos Google Scholar
Rodrigues, M. L. Google Scholar
Alviano, C. S. Google Scholar
Soares, RMD Google Scholar
Institution Universidade Federal do Rio de Janeiro (UFRJ)
Universidade Federal de São Paulo (UNIFESP)
Abstract The expression of sialoglycoconjugates during the dimethylsulfoxide (DMSO)-induced differentiation of Herpetomonas samuelpessotti was analyzed by flow cytometry and Western blotting using sialic acid-specific lectins. Parasites reacted strongly with Limax flavus (LFA) and Sambucus nigra (SNA) agglutinins, and only weakly with Maackia amurensis (MAA) lectin. However, analysis of crude protein extracts by Western blotting revealed that bands with molecular masses corresponding to 15 and 40 kDa are recognized by MAA, and that treatment with DMSO induced the expression of two additional polypeptides with molecular masses of 65 and 90 kDa. Profiles of binding to LFA were indistinguishable when protein extracts from control or differentiated cells were analyzed. SNA recognized a major molecule with 25 kDa in extracts from non-differentiate forms and two low-molecular-weight bands from differentiated cells. These results indicate that molecules containing alpha2,6 and alpha2,3 sialyl-galactosyl sequences are present in H. samuelpessoai, and that their biosynthesis and expression are influenced by DMSO-induced differentiation.
Language English
Date 2002-11-01
Published in Parasitology Research. New York: Springer-verlag, v. 88, n. 11, p. 951-955, 2002.
ISSN 0932-0113 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 951-955
Origin http://dx.doi.org/10.1007/s00436-002-0685-5
Access rights Closed access
Type Article
Web of Science ID WOS:000179019300001
URI http://repositorio.unifesp.br/handle/11600/27018

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