Expression in bacteria of the gene encoding the gp43 antigen of Paracoccidioides brasiliensis: Immunological reactivity of the recombinant fusion proteins

Expression in bacteria of the gene encoding the gp43 antigen of Paracoccidioides brasiliensis: Immunological reactivity of the recombinant fusion proteins

Autor Diniz, Susana N. Google Scholar
Carvalho, Katia C. Google Scholar
Cisalpino, Patricia S. Google Scholar
Silveira, Jose F. Google Scholar
Travassos, Luiz Rodolpho Autor UNIFESP Google Scholar
Puccia, Rosana Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo gp43 is the major diagnostic antigen of Paracoccidioides brasiliensis, the agent of paracoccidioidomycosis IPCM) in humans. in the present study, cDNA of the gp43 gene (PbGP43) was obtained by reverse transcriptase PCR, inserted into a pGEX vector in frame with the glutathione S-transferase (GST) gene, and expressed in Escherichia coli as inclusion bodies. Immunoblotting showed that all sera from patients with chronic pulmonary and acute lymphatic forms of PCM reacted with the recombinant fusion protein of the mature gp43 (381 amino acids). Reactivity with fusion proteins containing subfragments of the N-terminal, internal, or C-terminal regions occurred eventually, and the C-terminal region was the most antigenic. Lack of reactivity with the subfragments may be due to the conformational nature of the gp43 epitopes. Sera from patients with aspergillosis, candidiasis, and histoplasmosis did not react with the gp43-GST fusion protein. Our results suggest that recombinant gp43 corresponding to the processed antigen can be a useful tool in the diagnosis of PCM.
Idioma Inglês
Data de publicação 2002-11-01
Publicado em Clinical and Diagnostic Laboratory Immunology. Washington: Amer Soc Microbiology, v. 9, n. 6, p. 1200-1204, 2002.
ISSN 1071-412X (Sherpa/Romeo, fator de impacto)
Publicador Amer Soc Microbiology
Extensão 1200-1204
Fonte http://dx.doi.org/10.1128/CDLI.9.6.1200-1204.2002
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000179271100009
Endereço permanente http://repositorio.unifesp.br/handle/11600/27012

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