Molecular characterization of SPM-1, a novel metallo-beta-lactamase isolated in Latin America: report from the SENTRY antimicrobial surveillance programme

Molecular characterization of SPM-1, a novel metallo-beta-lactamase isolated in Latin America: report from the SENTRY antimicrobial surveillance programme

Autor Toleman, M. A. Google Scholar
Simm, A. M. Google Scholar
Murphy, T. A. Google Scholar
Gales, Ana C. Autor UNIFESP Google Scholar
Biedenbach, D. J. Google Scholar
Jones, R. N. Google Scholar
Walsh, T. R. Google Scholar
Instituição Univ Bristol
Universidade Federal de São Paulo (UNIFESP)
JONES Grp JMI Labs
Tufts Univ
Resumo The gene encoding the metallo-beta-lactamase SPM-1 was cloned from a genomic library of Pseudomonas aeruginosa strain 48-1997A. the insert carrying spm-1 possessed a GC content of 47%, indicating that it is of non-Pseudomonas origin. Upstream of spm-1 there is a small open reading frame (ORF), which is homologous to the LysR family of proteins (69% identity to the LysR protein from Salmonella enterica serovar Typhimurium). Downstream of spm-1 there is the start of an ORF, the product of which shows close homology with the GroEL-type proteins from Xanthomonas campestris. No transmissible element could be identified upstream or downstream of spm-1. the spm-1 gene is carried on a plasmid that can transform both Escherichia coli and P. aeruginosa to ceftazidime resistance. SPM-1 contains the classic metallo-beta-lactamase zinc-binding motif HXHXD and shows the highest identity (35.5%) to IMP-1. SPM-1 is a distinctly different metallo-beta-lactamase from VIM and IMP and, accordingly, represents a new subfamily of mobile metallo-beta-lactamases. the predicted molecular weight of the protein was 27515 Da, significantly higher than that of IMP (25041 Da) or VIM (25322 Da). SPM-1 possesses a unique loop of 23 residues that accounts for the higher molecular mass.
Palavra-chave metallo-beta-lactamase
SENTRY
Pseudomonas aeruginosa
Idioma Inglês
Data de publicação 2002-11-01
Publicado em Journal of Antimicrobial Chemotherapy. Oxford: Oxford Univ Press, v. 50, n. 5, p. 673-679, 2002.
ISSN 0305-7453 (Sherpa/Romeo, fator de impacto)
Publicador Oxford Univ Press
Extensão 673-679
Fonte http://dx.doi.org/10.1093/jac/dkf210
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000179034900008
Endereço permanente http://repositorio.unifesp.br/handle/11600/27010

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