Enzyme release from injured, preserved, and ex vivo reperfused liver does not indicate malfunction

Enzyme release from injured, preserved, and ex vivo reperfused liver does not indicate malfunction

Autor Gioli-Pereira, L. Google Scholar
Coradin, K. Google Scholar
Nagaoka, M. R. Google Scholar
Borges, D. R. Google Scholar
Kouyoumdjian, M. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Objective. We compared the enzyme release from preserved and ex vivo reperfused livers after acute injury or inflammatory stimulus with organ function.Methods. Acute injury was induced by carbon tetrachloride and inflammation was induced by turpentine oil treatments. Livers were exsanguinated and preserved for 8 or 24 hr. Enzymes were measured in preservation and reperfusion solutions, and reperfused liver function was evaluated by O-2 consumption and bromsulphalein clearance.Results. the release of lysosomal enzymes was negligible in the preservation solution, and that of alanine aminotransferase and lactate dehydrogenase was similar in all groups. Release of aspartate aminotransferase and of EC 3.4.24.15 was more than that of the controls. During reperfusion liver function was normal in the injured group.Conclusion. Release of enzymes, mainly aspartate aminotransferase and EC 3.4.24.15, into the preservation solution is a sensitive and early indicator of either inflammatory or acute injury alterations of the preserved liver, but does not reflect organ malfunction.
Idioma Inglês
Data de publicação 2002-10-27
Publicado em Transplantation. Philadelphia: Lippincott Williams & Wilkins, v. 74, n. 8, p. 1081-1083, 2002.
ISSN 0041-1337 (Sherpa/Romeo, fator de impacto)
Publicador Lippincott Williams & Wilkins
Extensão 1081-1083
Fonte http://dx.doi.org/10.1097/00007890-200210270-00004
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000178918400004
Endereço permanente http://repositorio.unifesp.br/handle/11600/27005

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