L-arginine effects on blood pressure and renal function of intrauterine restricted rats

L-arginine effects on blood pressure and renal function of intrauterine restricted rats

Author Alves, G. M. Google Scholar
Barao, M. A. Google Scholar
Odo, L. N. Google Scholar
Gomes, G. N. Google Scholar
Franco, MDP Google Scholar
Nigro, D. Google Scholar
Lucas, SRR Google Scholar
Laurindo, FRM Google Scholar
Brandizzi, LIV Google Scholar
Gil, F. Z. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract We have previously demonstrated that 3-month-old rats submitted to 50% intrauterine food restriction showed a decreased number of nephrons with increased glomerular diameter, a fact that suggests compensatory hypertrophy. in the present study, we extended the investigation and performed serial blood pressure measurements and renal function evaluation in 8- and 12-week-old rats submitted to 50% intrauterine food restriction (groups R8 and R12) and in age-matched control rats (groups C8 and C12). After weaning, six to eight animals from each group received oral supplements of 2% L-arginine (L-arg) solution for 4 or 8 weeks (groups CA8, CA12, RA8, RA12). Our findings showed that mean blood pressure (MBP), which was significantly increased from 8 weeks on in R rats, markedly decreased after L-arg supplementation. in control animals, no alterations in MBP were observed with L-arg. Proteinuria was within normal limits in all groups studied but L-arg caused a significant decrease in this parameter in both the RA8 and RA12 groups. Glomerular filtration rate (GFR, ml/min per kg) was significantly decreased in the C8 control group (3.75+/-0.12) and in both restricted groups R8 and R12, (2.47+/-0.13 and 3.76+/-0.16, respectively) compared with the C12 group (6.09+/-0.31; P<0.05 for all comparisons). L-Arg caused an increase in GFR only in the younger groups, C8 and R8. in a separate set of experiments., acetylcholine (ACh)-induced relaxation was examined in mesenteric arteries. the R12 group showed a significant impairment of the response to ACh, which returned to normal values after L-arg supplementation. Urinary excretion of NOX (NO3- + NO2-) was significantly decreased in 8- and 12-week-old food-restricted rats relative to control rats. Our data indicate that, besides the known decrease in absolute nephron number, disturbances in the production/sensitivity to the L-arg-nitric oxide system may contribute to the early appearance of hypertension in the offspring of mothers submitted to significant food restriction.
Keywords intrauterine undernutrition
nitric oxide
renal function
Language English
Date 2002-10-01
Published in Pediatric Nephrology. New York: Springer-verlag, v. 17, n. 10, p. 856-862, 2002.
ISSN 0931-041X (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 856-862
Origin http://dx.doi.org/10.1007/s00467-002-0941-z
Access rights Closed access
Type Article
Web of Science ID WOS:000179164100015
URI http://repositorio.unifesp.br/handle/11600/26990

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