Annexin 1 localisation in tissue eosinophils as detected by electron microscopy

Annexin 1 localisation in tissue eosinophils as detected by electron microscopy

Autor Oliani, Sonia Maria Autor UNIFESP Google Scholar
Damazo, Amilcar Sabino Autor UNIFESP Google Scholar
Perretti, M. Google Scholar
Instituição Barts & London Queen Marys Sch Med & Dent
Universidade Federal de São Paulo (UNIFESP)
Resumo BACKGROUND: Human and rodent leukocytes express high levels of the glucocorticoid-inducible protein annexin 1 ( ANXA1) ( previously referred to as lipocortin 1). Neutrophils and monocytes have abundant ANXA1 levels.Aim: We have investigated, for the first time, ANXA1 ultrastructural expression in rat eosinophils and compared it with that of extravasated neutrophils. the effect of inflammation ( carrageenin peritonitis) was also monitored.Methods: Electron microscopy was used to define the sub-cellular localisation of ANXA1 in rat eosinophils and neutrophils extravasated in the mesenteric tissue. A pair of antibodies raised against the ANXA1 N-terminus (i.e. able to recognise intact ANXA1, termed LCPS1) or the whole protein ( termed LCS3) was used to perform the ultrastructural analysis.Results: the majority of ANXA1 was localised in the eosinophil cytosol (similar to 60%) and nucleus (30-40%), whereas a small percentage was found on the plasma membrane (< 10%). Within the cytosol, the protein was equally distributed in the matrix and in the granules, including those containing the typical crystalloid. the two anti-ANXA1 antibodies gave similar results, with the exception that LCPS1 gave a lower degree of immunoreactivity in the plasma membrane. Inflammation (i.e. carrageenin injection) produced a modest increase in eosinophil-associated ANXA1 reactivity ( significant only in the cytoplasm compartment). Extravasated neutrophils, used for comparative purposes, displayed a much higher degree of immunoreactivity for the protein.Conclusion: We describe for the first time ANXA1 distribution in rat eosinophil by ultrastructural analysis, and report a different protein mobilisation from extravasated neutrophils, at least in this acute model of peritonitis.
Assunto neutrophil
lipocortin 1
Idioma Inglês
Data 2002-10-01
Publicado em Mediators of Inflammation. New York: Hindawi Publishing Corporation, v. 11, n. 5, p. 287-292, 2002.
ISSN 0962-9351 (Sherpa/Romeo, fator de impacto)
Editor Hindawi Publishing Corporation
Extensão 287-292
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000179222100002

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