Glycosphingolipids from Magnaporthe grisea cells: expression of a ceramide dihexoside presenting phytosphingosine as the long-chain base

Glycosphingolipids from Magnaporthe grisea cells: expression of a ceramide dihexoside presenting phytosphingosine as the long-chain base

Autor Maciel, D. M. Google Scholar
Rodrigues, M. L. Google Scholar
Wait, R. Google Scholar
Boas, MHSV Google Scholar
Tischer, C. A. Google Scholar
Barreto-Bergter, E. Google Scholar
Instituição Universidade Federal do Rio de Janeiro (UFRJ)
Universidade Federal de São Paulo (UNIFESP)
Ctr Appl Microbiol & Res
INCQS
Univ Fed Parana
Resumo Magnaporthe grisea is a fungal pathogen that infects rice leaves and causes rice blast, a devastating crop disease. W. grisea produces active elicitors of the hypersensitive response in rice that were previously identified as ceramide monohexosides (CMHs). Using several chromatographic approaches, mass spectrometry, and nuclear magnetic resonance, we identified ceramide mono- and dihexosides (CDH) in purified lipid extracts from M. grisea cells. As described by other authors, CMH consists of a ceramide moiety containing 9-methyl-4,8-sphingadienine in amidic linkage to 2-hydroxyoctadecenoic or 2-hydroxyhexadecenoic acids and a carbohydrate segment consisting of one residue of glucose. CDHs, however, contain beta-galactose (1 --> 4)-linked to beta-glucose as sugar units and phytosphingosine as the long-chain base, bound to a C24 alpha-hydroxylated fatty acid. To our knowledge, this is the first report on the occurrence of CDH in a fungal species and illustrates the existence of an alternative path of ceramide glycosylation in fungal cells. (C) 2002 Elsevier Science (USA). All rights reserved.
Palavra-chave Magnaporthe grisea
glucosylceramide
phytosphingosine
ceramide dihexoside
Idioma Inglês
Data de publicação 2002-09-15
Publicado em Archives of Biochemistry and Biophysics. San Diego: Academic Press Inc Elsevier Science, v. 405, n. 2, p. 205-213, 2002.
ISSN 0003-9861 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 205-213
Fonte http://dx.doi.org/10.1016/S0003-9861(02)00365-X
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000178283500009
Endereço permanente http://repositorio.unifesp.br/handle/11600/26980

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