Disruption of the glucosylceramide biosynthetic pathway in Aspergillus nidulans and Aspergillus fumigatus by inhibitors of UDP-Glc : ceramide glucosyltransferase strongly affects spore germination, cell cycle, and hyphal growth

Disruption of the glucosylceramide biosynthetic pathway in Aspergillus nidulans and Aspergillus fumigatus by inhibitors of UDP-Glc : ceramide glucosyltransferase strongly affects spore germination, cell cycle, and hyphal growth

Autor Levery, S. B. Google Scholar
Momany, M. Google Scholar
Lindsey, R. Google Scholar
Toledo, Marcos Sergio de Autor UNIFESP Google Scholar
Shayman, J. A. Google Scholar
Fuller, M. Google Scholar
Brooks, K. Google Scholar
Doong, R. L. Google Scholar
Straus, Anita Hilda Autor UNIFESP Google Scholar
Takahashi, Helio Kiyoshi Autor UNIFESP Google Scholar
Instituição Univ New Hampshire
Univ Georgia
Universidade Federal de São Paulo (UNIFESP)
Univ Michigan
Resumo The opportunistic mycopathogen Aspergillus fumigatus expresses both glucosylceramide and galactosylceramide (GlcCer and GalCer), but their functional significance in Aspergillus species is unknown. We here identified and characterized a GlcCer from Aspergillus nidulans, a non-pathogenic model fungus. Involvement of GlcCer in fungal development was tested on both species using a family of compounds known to inhibit GlcCer synthase in mammals. Two analogs, D-threo-1-phenyl-2-palmitoyl-3-pyrrolidinopropanol (P4) and D-threo-3',4'-ethylenedioxy-P4, strongly inhibited germination and hyphal growth. Neutral lipids from A. fumigatus cultured in the presence of these inhibitors displayed a significantly reduced GlcCer/GalCer ratio. These results suggest that synthesis of GlcCer is essential for normal development of A. fumigatus and A. nidulans. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
Palavra-chave sphingolipid
fungus
glycosyltransferase
glycolipid
PDMP
synthase
Idioma Inglês
Data de publicação 2002-08-14
Publicado em Febs Letters. Amsterdam: Elsevier B.V., v. 525, n. 1-3, p. 59-64, 2002.
ISSN 0014-5793 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 59-64
Fonte http://dx.doi.org/10.1016/S0014-5793(02)03067-3
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000177481000013
Endereço permanente http://repositorio.unifesp.br/handle/11600/26946

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