Role of preoptic second messenger systems (cAMP and cGMP) in the febrile response

Role of preoptic second messenger systems (cAMP and cGMP) in the febrile response

Autor Steiner, A. A. Google Scholar
Antunes-Rodrigues, J. Google Scholar
Branco, LGS Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Dent Sch Ribeirao Preto
Resumo The present study aimed to test the hypothesis that a decrease in preoptic cAMP mediates fever. To this end, body core temperature (T-c) of unanesthetized, freely moving rats was monitored by biotelemetry before and after pharmacological modulation of the cAMP pathway, and cAMP levels in the anteroventral third ventricular region (AV3V), where the preoptic region (POA) is located, were determined. We observed that intra-POA administration of the cAMP agonist dibutyryl-cAMP (Db-cAMP, 40 mug) reduced T-c. PGE(2) (the proximal mediator of fever, 200 ng) raised T-c with a concomitant decrease in AV3V cAMP levels from 22.7+/-1.8 to 17.0+/-1.0 fmol/mug protein. Moreover, PGE(2)-induced fever was impaired by the phosphodiesterase inhibitor aminophylline. in order to verify the interaction between the cAMP- and cGMP-dependent pathways in the POA, we then co-injected Db-cAMP and 8-Br-cGMP into the POA. As a result, 8-Br-cGMP augmented the drop in 7 evoked by Db-cAMP. Lastly, we observed that intra-POA co-microinjection of the protein kinase A inhibitor (Rp-cAMPS, 1 mug) with the protein kinase G inhibitor (Rp-cGMPS, 1 mug), mimicking the effects of reduced production of cAMP and cGMP, respectively, produced a fever-like response. in summary, the present data support that a decrease in the levels of cAMP and cGMP in the POA is associated with the genesis of fever. (C) 2002 Elsevier Science BY. All rights reserved.
Assunto body temperature
nitric oxide
set point
temperature-sensitive neuron
cyclic AMP
cyclic GMP
Idioma Inglês
Data 2002-07-19
Publicado em Brain Research. Amsterdam: Elsevier B.V., v. 944, n. 1-2, p. 135-145, 2002.
ISSN 0006-8993 (Sherpa/Romeo, fator de impacto)
Editor Elsevier B.V.
Extensão 135-145
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000177224100014

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