Biochemical and cytochemical characterization of extracellular proteoglycans in the inner circular smooth muscle layer of dog small intestine

Biochemical and cytochemical characterization of extracellular proteoglycans in the inner circular smooth muscle layer of dog small intestine

Autor Toledo, Olga Maria Szymanski de Autor UNIFESP Google Scholar
Marquezini, Monica Valeria Google Scholar
Jia, Kaio Bin Autor UNIFESP Google Scholar
Pinheiro, M. D. Google Scholar
Mora, Oswaldo Alves Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Prosangue Fdn Hemoctr São Paulo
Universidade Estadual de Campinas (UNICAMP)
Resumo Current literature concerning smooth muscle blood vessels has shown versican as the main proteoglycan (PG) component of the matrix. To show whether smooth muscle matrix has the same PG distribution when present in organs, other than the blood vessels, the inner circular smooth muscle layer of the small intestine was obtained by dissection as a highly purified tissue and analyzed by biochemical and cytochemical methods. the smooth muscle layer PGs were extracted from dog small intestine with 4 M guanidine-HCl in the presence of proteinase inhibitors, purified by charge equilibrium, isolated by equilibrium CsCl density gradients, and analyzed in terms of anion exchange, size, and glycosaminoglycan (GAG) distribution. Proteoheparan sulfate itself represented 91.5% of the PGs present in this tissue. the remainder was proteodermatan sulfate. Cytochemical analyses using the cationic dye cuprolinic blue associated with enzymatic treatments with chondroitinases ABC and heparitinase III showed the arrangement and identification of PGs in basal lamina and intramuscular connective tissues. the PGs in the basal lamina were proteoheparan sulfate, and those associated with collagen fibrils in the endomysium and perimysium were rich in dermatan sulfate. in contrast to the blood vessels, inner circular muscle smooth tissue in intestine has, as the main PG, perlecan.
Palavra-chave dermatan sulfate
intestine
PG
proteoheparan sulfate
smooth muscle
Idioma Inglês
Data de publicação 2002-07-01
Publicado em Iubmb Life. Philadelphia: Taylor & Francis Inc, v. 54, n. 1, p. 19-25, 2002.
ISSN 1521-6543 (Sherpa/Romeo, fator de impacto)
Publicador Taylor & Francis Inc
Extensão 19-25
Fonte http://dx.doi.org/10.1080/15216540213824
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000178079600004
Endereço permanente http://repositorio.unifesp.br/handle/11600/26922

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