Prevalence of mutations related to HIV-1 antiretroviral resistance in Brazilian patients failing HAART

Prevalence of mutations related to HIV-1 antiretroviral resistance in Brazilian patients failing HAART

Autor Tanuri, A. Google Scholar
Caridea, E. Google Scholar
Dantas, M. C. Google Scholar
Morgado, M. G. Google Scholar
Mello, DLC Google Scholar
Borges, S. Google Scholar
Tavares, M. Google Scholar
Ferreira, S. B. Google Scholar
Santoro-Lopes, G. Google Scholar
Martins, CRF Google Scholar
Esteves, ALC Google Scholar
Diaz, R. S. Google Scholar
Andreo, SMS Google Scholar
Ferreira, LAP Google Scholar
Rodrigues, R. Google Scholar
Reuter, T. Google Scholar
Cavalcanti, AMS Google Scholar
Oliveira, S. M. de Google Scholar
Barbosa, H. B. de Google Scholar
Teixeira, P. R. Google Scholar
Chequer, P. N. Google Scholar
Instituição Universidade Federal do Rio de Janeiro (UFRJ)
Brazilian Minist Hlth
Fiocruz MS
Univ Fed Espirito Santos
Universidade de Brasília (UnB)
Lab Cent Saude Publ
Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Universidade Federal de Santa Catarina (UFSC)
Resumo Background: Current guidelines for antiretroviral (ARV) therapy recommend at least triple-drug combination, the so-called highly active antiretroviral therapy (HAART). Not all patients respond to HAART and the development of drug resistance remains one of the most serious obstacles to sustained suppression of HIV. Objective: in an attempt to correlate the HIV therapeutic failure with reverse transcriptase (RT) and protease resistance mutations, we describe the ARV resistance profile in patients failing HAART in Brazil. We studied 267 Brazilian HIV-1 infected patients failing HAART looking for mutations in RT and protease genes. the mutation profile of the viruses infecting these individuals were deduced and correlated to laboratorial parameters. Study Design: Two different HIV-1 genomic regions were targeted for PCR amplification, the protease (pro) and pal RT (palm finger region) genes. the mutations related to drug resistance in RT gene was analyzed using a line probe assay (LIPA(R)) and pro amino acids positions 82 and 90 were screened through RFLP using HincII restriction digestion. Results: There was strong correlation between the mutation in the pro and RT genes and therapeutic failure. the main mutation found in RT gene was the M184V (48%) followed by T69D/N (47%), T215Y/F (46%), M41L (39%), and L74V (7%). in the pro gene the main mutation found was L90M (26%) followed by dual substitution in L90M and V82A (6%). All mutations profiles matched very well with the patients drug regimen. Conclusions: This study has shown that 84.7% of HIV infected subjects failing HAART for more than 3 months presented viral genomic mutations associated with drug resistance. (C) 2002 Elsevier Science B.V. All rights reserved.
Palavra-chave HIV-1
protease
reverse transcriptase
phenotyping
genotyping
Idioma Inglês
Data de publicação 2002-07-01
Publicado em Journal of Clinical Virology. Amsterdam: Elsevier B.V., v. 25, n. 1, p. 39-46, 2002.
ISSN 1386-6532 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 39-46
Fonte http://dx.doi.org/10.1016/S1386-6532(01)00249-9
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000177400900005
Endereço permanente http://repositorio.unifesp.br/handle/11600/26913

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