Stress-inducible protein 1 is a cell surface ligand for cellular prion that triggers neuroprotection

Stress-inducible protein 1 is a cell surface ligand for cellular prion that triggers neuroprotection

Autor Zanata, S. M. Google Scholar
Lopes, M. H. Google Scholar
Mercadante, A. F. Google Scholar
Hajj, GNM Google Scholar
Chiarini, L. B. Google Scholar
Nomizo, R. Google Scholar
Freitas, ARO Google Scholar
Cabral, ALB Google Scholar
Lee, K. S. Google Scholar
Juliano, Maria A. Autor UNIFESP Google Scholar
Oliveira, E. de Google Scholar
Jachieri, S. G. Google Scholar
Burlingame, A. Google Scholar
Huang, L. Google Scholar
Linden, R. Google Scholar
Brentani, R. R. Google Scholar
Martins, V. R. Google Scholar
Instituição Ludwig Inst Canc Res
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Universidade Federal do Rio de Janeiro (UFRJ)
USCF
Resumo Prions are composed of an isoform of a normal sialoglycoprotein called PrPc, whose physiological role has been under investigation, with focus on the screening for ligands. Our group described a membrane 66 kDa PrPc-binding protein with the aid of antibodies against a peptide deduced by complementary hydropathy. Using these antibodies in western blots from two-dimensional protein gels followed by sequencing the specific spot, we have now identified the molecule as stress-inducible protein 1 (STI1). We show that this protein is also found at the cell membrane besides the cytoplasm. Both proteins interact in a specific and high affinity manner with a K-d of 10(-7) M. the interaction sites were mapped to amino acids 113-128 from PrPc and 230-245 from STI1. Cell surface binding and pull-down experiments showed that recombinant PrPc binds to cellular STI1, and co-immunoprecipitation assays strongly suggest that both proteins are associated in vivo. Moreover, PrPc interaction with either STI1 or with the peptide we found that represents the binding domain in STI1 induce neuropro tective signals that rescue cells from apoptosis.
Palavra-chave neuroprotection
prion
PrPc
PrPc ligand
stress-inducible protein 1
Idioma Inglês
Data de publicação 2002-07-01
Publicado em Embo Journal. Oxford: Oxford Univ Press, v. 21, n. 13, p. 3307-3316, 2002.
ISSN 0261-4189 (Sherpa/Romeo, fator de impacto)
Publicador Oxford Univ Press
Extensão 3307-3316
Fonte http://dx.doi.org/10.1093/emboj/cdf325
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000176784100010
Endereço permanente http://repositorio.unifesp.br/handle/11600/26902

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