In vitro selection of RNA aptamers that bind to cell adhesion receptors of Trypanosoma cruzi and inhibit cell invasion

In vitro selection of RNA aptamers that bind to cell adhesion receptors of Trypanosoma cruzi and inhibit cell invasion

Autor Ulrich, Alexander Henning Autor UNIFESP Google Scholar
Magdesian, M. H. Google Scholar
Alves, MJM Google Scholar
Colli, W. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Trypanosoma cruzi causing Chagas' disease needs to invade host cells to complete its life cycle. Macromolecules on host cell surfaces such as laminin, thrombospondin, heparan sulfate, and fibronectin are believed to be important in mediating parasite-host cell adhesions and in the invasion process of the host cell by the parasite. the SELEX technique (systematic evolution of ligands bygxponential enrichment) was used to evolve nuclease-resistant RNA ligands (aptamer = to fit) that bind with affinities of 40-400 nm to parasite receptors for the host cell matrix molecules laminin, fibronectin, thrombospondin, and heparan sulfate. After eight consecutive rounds of in vitro selection four classes of RNA aptamers based on structural similarities were isolated and sequenced. All members of each class shared a common sequence motif and competed with the respective host cell matrix molecule that was used for displacement during the selection procedure. RNA pools following seven and eight selection rounds as well as individual aptamers sharing consensus motifs were active in inhibiting invasion of LLC-MK(2) monkey kidney cells by T. cruzi in vitro.
Idioma Inglês
Data de publicação 2002-06-07
Publicado em Journal of Biological Chemistry. Bethesda: Amer Soc Biochemistry Molecular Biology Inc, v. 277, n. 23, p. 20756-20762, 2002.
ISSN 0021-9258 (Sherpa/Romeo, fator de impacto)
Publicador Amer Soc Biochemistry Molecular Biology Inc
Extensão 20756-20762
Fonte http://dx.doi.org/10.1074/jbc.M111859200
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000176204500085
Endereço permanente http://repositorio.unifesp.br/handle/11600/26895

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