Bradykinin induces a calcium-store-dependent calcium influx in mouse mesangial cells

Bradykinin induces a calcium-store-dependent calcium influx in mouse mesangial cells

Author Campos, A. H. Google Scholar
Calixto, J. B. Google Scholar
Schor, N. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade Federal de Santa Catarina (UFSC)
Abstract Bradykinin (BK) elicits extracellular-dependent [Ca2+]i elevations in mouse mesangial cells (MMC) that are not blocked by verapamil, nifeclipine, L-nicardipine, NiCl2, or LaCl3. the aim of the present study was to evaluate the mechanisms involved in calcium influx induced by BK in MMC. [Ca2+](i) was analyzed through spectrofluorometry employing fura-2-AM, and the data were expressed as [Ca2+](i) obtained/[Ca2+](i) basal ratio. Heparin (IP3, a receptor antagonist) almost abolished the effects of BK in MMC (1.85 +/- 0.15 vs. 1.13 +/- 0.02, n = 4, p = 0.001). Following external and intracellular calcium store depletion, BK's effect was absent even after successful extracellular calcium replenishment. ML-7 (a myosin light chain kinase inhibitor) blocked responses to thapsigargin (2.62 +/- 0.13 vs. 1.11 +/- 0.04, n = 4, p < 0.001), but not those of BK (6.51 +/- 0.39, n = 6, vs. 5.86 +/- 1.17, n = 4, p = 0.39). On the other hand, genistein (a tyrosine kinase inhibitor) was able to inhibit thapsigargin (3.12 +/- 0.22, n = 5, vs. 1.28 +/- 0.16, n = 4, p < 0.001) as well as BK responses (6.46 +/- 0.66 vs. 2.89 +/- 0.61, n = 4, p < 0.05). Econazole (a P-450 monooxygenase inhibitor) inhibited the responses to both thapsigargin (3.45 +/- 0.16 vs. 1.03 +/- 0.03, n = 4, p < 0.001) and BK (6.49 +/- 0.83, n = 6, vs. 1.17 +/- 0.08, n = 4, p = 0.01). Finally, responses to BK were not affected by indomethacin (6.69 +/- 0.66 vs. 6.57 +/- 0.87, n = 4, p = 0.916). Thus, BK promotes an IP3-sensitive store-dependent calcium influx in MMC. This phenomenon seems to involve tyrosine kinase and P-450 monooxygenase products in its transduction pathway. Copyright (C) 2002 S. KargerAG, Basel.
Keywords bradykinin
mouse mesangial cells
calcium-store-dependent operated channels
Language English
Date 2002-06-01
Published in Nephron. Basel: Karger, v. 91, n. 2, p. 308-315, 2002.
ISSN 0028-2766 (Sherpa/Romeo, impact factor)
Publisher Karger
Extent 308-315
Origin http://dx.doi.org/10.1159/000058409
Access rights Closed access
Type Article
Web of Science ID WOS:000176414800021
URI http://repositorio.unifesp.br/handle/11600/26894

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