Up-regulation of Ca2+ channels in vas deferens after chronic treatment of newborn rats with nifedipine

Up-regulation of Ca2+ channels in vas deferens after chronic treatment of newborn rats with nifedipine

Autor Verde, L. F. Google Scholar
Lafayette, SSL Google Scholar
Caricati-Neto, A. Google Scholar
Jurkiewicz, N. H. Google Scholar
Jurkiewicz, A. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Radioligand binding and contraction techniques were used to verify if L-type Ca2+ charnels are modified in rat vas deferens after treatment with the blocker nifedipine (15 mug), injected at 7, 14, 21 and 28 days after birth. Vas deferens tissue was used 10, 30 and 90 days after the last injection, to verify if modifications are persistent. Binding studies with cell membranes, using [H-3]isradipine, showed an increase of the density (B-max) of Ca2+ channels by more than 60%, after 10 and 30 days, without changes of affinity (K-d). Maximal contractions (E-max) of KCl, were increased by 106% and 37%, respectively, after 10 and 30 days, without changes of apparent affinity (pD(2)). After 90 days, the values of B-max, K-d, E-max and pD(2) were not different from the controls. Differences were also not found for rats injected when adult. It is concluded that treatment of newborn, but not of adult, rats with nifedipine produced a long-lasting, though reversible, upregulation of L-type Ca2+ channels. (C) 2002 Elsevier Science B.V. All rights reserved.
Assunto Ca2+ channel
nifedipine
smooth muscle
contraction
binding
vas deferens
rat
Idioma Inglês
Data 2002-05-17
Publicado em European Journal of Pharmacology. Amsterdam: Elsevier B.V., v. 443, n. 1-3, p. 13-18, 2002.
ISSN 0014-2999 (Sherpa/Romeo, fator de impacto)
Editor Elsevier B.V.
Extensão 13-18
Fonte http://dx.doi.org/10.1016/S0014-2999(02)01584-4
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000176293500003
URI http://repositorio.unifesp.br/handle/11600/26872

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