Contractile responses of the rat vas deferens after epithelium removal

Contractile responses of the rat vas deferens after epithelium removal

Author Okpalaugo, E. O. Google Scholar
Garcez-do-Carmo, L. Google Scholar
Jurkiewicz, N. H. Google Scholar
Jurkiewicz, A. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract The purpose of the present investigation was to verify the role of the epithelium in the functional response of the rat vas deferens. Our results showed that the contractile effect of cumulative doses of clonidine (3.10(-5) -3.10(-3)) was increased after the removal of the epithelium. the effect of clonidine in epithelium-free vas deferens returned to normal values when an isolated epithelium from another vas deferens was added to the organ bath, showing that the epithelium is responsible for this increase of maximum effect for clonidine. Drugs functionally or structurally related to clonidine, such as oxymetazoline, alpha-methylnorepinephrine and moxonidine, did not have their dose-response curves altered. the curves for other contractile agents, such as noradrenaline, acetylcholine, ATP, 5HT, bradykinin and histamine, or the relaxation induced by isoprenaline and forskolin were also not modified. Electrically-induced contractions at frequencies from 0.1 to 20 Hz and the mechanism of negative feed-back, brought about by clonidine (10(-10)-10(-8) M) through pre-synaptic alpha(2)-adrenoceptors, were not changed after the removal of epithelium. in conclusion, a significant function of the epithelium in the contractility of the rat vas deferens was demonstrated for clonidine, but not for other agonists. (C) 2002 Elsevier Science Inc. All rights reserved.
Keywords epithelium
rat vas deferens
clonidine
Language English
Date 2002-05-03
Published in Life Sciences. Oxford: Pergamon-Elsevier B.V., v. 70, n. 24, p. 2943-2951, 2002.
ISSN 0024-3205 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 2943-2951
Origin http://dx.doi.org/10.1016/S0024-3205(02)01545-X
Access rights Closed access
Type Article
Web of Science ID WOS:000176374800008
URI http://repositorio.unifesp.br/handle/11600/26869

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