Protein kinase C modulators enhance angiotensin II desensitization of guinea pig ileum via maxi-K+ channels

Protein kinase C modulators enhance angiotensin II desensitization of guinea pig ileum via maxi-K+ channels

Autor Aboulafia, J. Google Scholar
Silva, B. A. Google Scholar
Nouailhetas, VLA Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo We investigated the role of protein kinase in the desensitization of the angiotensin II induced contraction of guinea pig ileum. in contrast to their antagonistic effects on enzymatic activity, body activation blockers accelerated the dissipation of the 10(-7) M angiotensin II isometric contractile response. These agents indirectly activated maxi-K- channels in cell-attached membrane patches from freshly dispersed myocytes bathed in high-K- solution and clamped at -40 mV. in parallel with the contractile responses, fura 2-loaded myocytes bathed in Tyrode solution showed additive increases in [Ca2+] in response to both angiotensin II and phorbol dibutyrate (PDB). the PDB-promoted increase of the rate of angiotensin II desensitization was completely abolished by pretreatment of the tissue strips with 93 nM iberiotoxin or 8 mM KCl. Thus, we conclude that protein kinase C modulators promote faster angiotensin II desensitization by recruiting maxi-K- channels and inducing membrane repolarization rather than by affecting the protein kinase C activity. (C) 2002 Elsevier Science B.V. All rights reserved.
Palavra-chave angiotensin II desensitization
phorbol dibutyrate (PDB)
membrane repolarization
protein kinase C inhibitor
maxi-K- channel recruitment
ileum, guinea pig
Idioma Inglês
Data de publicação 2002-05-03
Publicado em European Journal of Pharmacology. Amsterdam: Elsevier B.V., v. 442, n. 1-2, p. 29-36, 2002.
ISSN 0014-2999 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 29-36
Fonte http://dx.doi.org/10.1016/S0014-2999(02)01500-5
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000176080900004
Endereço permanente http://repositorio.unifesp.br/handle/11600/26867

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