Role of the Gly40Ser mutation in the glucagon receptor gene in Brazilian patients with type 2 diabetes mellitus

Role of the Gly40Ser mutation in the glucagon receptor gene in Brazilian patients with type 2 diabetes mellitus

Autor Shiota, D. Google Scholar
Kasamatsu, T. Google Scholar
Dib, S. A. Google Scholar
Chacra, A. R. Google Scholar
Moises, R. S. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Introduction: A missense mutation in the glucagon receptor gene (Gly40Ser) has been associated with type 2 diabetes mellitus in some populations.Aim: To investigate whether this mutation is associated with type 2 diabetes in Brazilian patients and its functional significance in vivo.Methodology: One hundred fifteen patients with type 2 diabetes and 115 control subjects were screened by restriction-enzyme digestion with BstE II. the in vivo implications were investigated by I mg glucagon intravenous injection and plasma C-peptide (before and after 6 minutes) and glucose measurements (before and after 30, 60, 90, and 120 minutes). and first-phase insulin response (1 + 3 minutes) to intravenous glucose tolerance test. These procedures were performed in two groups of patients with diabetes, which differed only by the presence or absence of the Gly40Ser mutation, and two groups of control subjects.Results: the mutation was detected in two patients with diabetes (1.7%) and in four control subjects (3.5%) (not significant). Patients with diabetes and carriers of Gly40Ser showed basal C-peptide levels significantly lower than noncarriers (0.70 ng/mL versus 1.50 ng/mL, p = 0.008). No differences were found between Gly40Ser carriers and noncarriers in control subjects within the parameters studied.Conclusion: Our results show that the Gly40Ser mutation in the glucagon receptor gene is not associated with type 2 diabetes in a Brazilian population. However, a reduction of insulin secretion was observed in Gly40Ser carriers.
Palavra-chave glucagon receptor
insulin secretion
type 2 diabetes
Idioma Inglês
Data de publicação 2002-05-01
Publicado em Pancreas. Philadelphia: Lippincott Williams & Wilkins, v. 24, n. 4, p. 386-390, 2002.
ISSN 0885-3177 (Sherpa/Romeo, fator de impacto)
Publicador Lippincott Williams & Wilkins
Extensão 386-390
Fonte http://dx.doi.org/10.1097/00006676-200205000-00010
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000175266300010
Endereço permanente http://repositorio.unifesp.br/handle/11600/26864

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