Estrogen regulation of uterine genes in vivo detected by complementary DNA array

Estrogen regulation of uterine genes in vivo detected by complementary DNA array

Autor Andrade, P. M. Google Scholar
Silva, IDCG Google Scholar
Borra, R. C. Google Scholar
Lima, G. R. de Google Scholar
Baracat, E. C. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Introduction: in the present study, our aim was to identify differentially expressed genes involved in estrogen actions at the endometrium level in rats. Methods: Thirty adult rats were ovariectomized four days prior to drug administration for 48 days. Rats were divided in 2 groups: I, control and II, conjugated equine estrogens (CCE). Total RNA was isolated from uterus, and differential expression was analyzed by array technology and RTPCR. Results: A total of 32 candidate genes were shown to be upregulated or downregulated in groups I or II. Among them, differential expression was already confirmed by RT-PCR for IGFBP5, S12, c-kit, and VEGF, genes whose expression was up regulated during CCE therapy, and casein kinase II and serine kinase expression was the same level in both groups. Conclusion: We have demonstrated that cDNA array represents a powerful approach to identify key molecules in the estrogens therapy. A number of the candidates reported here should provide new markers that may contribute to the detection of target estrogen receptor. This information may also aid the development of new approaches to therapeutic intervention.
Palavra-chave uterus
array
estrogen
rat
pharmacogenomic
Idioma Inglês
Data de publicação 2002-05-01
Publicado em Hormone and Metabolic Research. Stuttgart: Georg Thieme Verlag Kg, v. 34, n. 5, p. 238-244, 2002.
ISSN 0018-5043 (Sherpa/Romeo, fator de impacto)
Publicador Georg Thieme Verlag Kg
Extensão 238-244
Fonte http://dx.doi.org/10.1055/s-2002-32136
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000176376000004
Endereço permanente http://repositorio.unifesp.br/handle/11600/26859

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