Estrogen regulation of uterine genes in vivo detected by complementary DNA array

Estrogen regulation of uterine genes in vivo detected by complementary DNA array

Author Andrade, P. M. Google Scholar
Silva, IDCG Google Scholar
Borra, R. C. Google Scholar
Lima, G. R. de Google Scholar
Baracat, E. C. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Introduction: in the present study, our aim was to identify differentially expressed genes involved in estrogen actions at the endometrium level in rats. Methods: Thirty adult rats were ovariectomized four days prior to drug administration for 48 days. Rats were divided in 2 groups: I, control and II, conjugated equine estrogens (CCE). Total RNA was isolated from uterus, and differential expression was analyzed by array technology and RTPCR. Results: A total of 32 candidate genes were shown to be upregulated or downregulated in groups I or II. Among them, differential expression was already confirmed by RT-PCR for IGFBP5, S12, c-kit, and VEGF, genes whose expression was up regulated during CCE therapy, and casein kinase II and serine kinase expression was the same level in both groups. Conclusion: We have demonstrated that cDNA array represents a powerful approach to identify key molecules in the estrogens therapy. A number of the candidates reported here should provide new markers that may contribute to the detection of target estrogen receptor. This information may also aid the development of new approaches to therapeutic intervention.
Keywords uterus
array
estrogen
rat
pharmacogenomic
Language English
Date 2002-05-01
Published in Hormone and Metabolic Research. Stuttgart: Georg Thieme Verlag Kg, v. 34, n. 5, p. 238-244, 2002.
ISSN 0018-5043 (Sherpa/Romeo, impact factor)
Publisher Georg Thieme Verlag Kg
Extent 238-244
Origin http://dx.doi.org/10.1055/s-2002-32136
Access rights Closed access
Type Article
Web of Science ID WOS:000176376000004
URI http://repositorio.unifesp.br/handle/11600/26859

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