Comparison of DNA hybridization and PCR assays for detection of putative pathogenic enteroadherent Escherichia coli

Comparison of DNA hybridization and PCR assays for detection of putative pathogenic enteroadherent Escherichia coli

Autor Scaletsky, Isabel CA Autor UNIFESP Google Scholar
Fabbricotti, Sandra H. Autor UNIFESP Google Scholar
Aranda, Katia R. Autor UNIFESP Google Scholar
Morais, Mauro B. Autor UNIFESP Google Scholar
Fagundes-Neto, Ulysses Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo The correlation of the different adherence patterns with DNA probes and PCR primers for the identification of Escherichia coli was analyzed in isolates from children, less than 2 years of age with or without diarrhea, from different regions of Brazil. A total of 1,428 isolates obtained from 338 patients and 322 control children were studied. the enteropathogenic E. coli (EPEC) adherence factor (EAF) probe was shown to be as good as the HEp-2 adhesion assay for the detection of typical EPEC strains. the DNA probes used to detect diffusely adhering E. coli and enteroaggregative E. coli (EAEC) showed low sensitivities (64 and 50%, respectively), and the best method of identifying these organisms in clinical research remains the HEp-2 adherence assay. the bundle-forming pilus (BFP) and the EAEC PCR assays could be used instead of the DNA probes as a screening method for typical EPEC and EAEC carrying the EAEC probe sequence in the clinical laboratory. in our study, only typical EPEC strains that carried EAF and BFP were associated with acute diarrhea.
Idioma Inglês
Data de publicação 2002-04-01
Publicado em Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 40, n. 4, p. 1254-1258, 2002.
ISSN 0095-1137 (Sherpa/Romeo, fator de impacto)
Publicador Amer Soc Microbiology
Extensão 1254-1258
Fonte http://dx.doi.org/10.1128/JCM.40.4.1254-1258.2002
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000174808000022
Endereço permanente http://repositorio.unifesp.br/handle/11600/26809

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