Evidence for changes in the tachyphylactic property of recombinant angiotensin II AT(1) receptor expressed in CHO cells

Evidence for changes in the tachyphylactic property of recombinant angiotensin II AT(1) receptor expressed in CHO cells

Autor Franca, L. P. Google Scholar
Corrêa, Silvana Aparecida Alves Autor UNIFESP Google Scholar
Barbosa, AMRB Google Scholar
Ferreira, A. T. Google Scholar
Han, S. W. Google Scholar
Shimuta, S. I. Google Scholar
Paiva, ACM Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo The manifestation of tachyphylaxis to angiotensin 11 in Chinese hamster ovary (CHO) cells expressing the rat angiotensin II AT(1) receptor was investigated. the cells were transfected with a cDNA fragment containing the complete coding region of the angiotensin II AT(1A) receptor gene, as well as 56 bp of its 3'- and 52 bp of its 5-untranslated regions. These cells (CHO-AT(1)) responded to angiotensin 11 by increases in intracellular Ca2+ concentration and inositol phosphate turnover, which were inhibited upon repeated administrations, characterizing the tachyphylaxis phenomenon. in contrast to smooth muscle cells, which are rendered tachyphylactic to angiotensin H but not to [2-lysine]angiotensin 11 ([Lys(2)]angiotensin 11), this analogue induced responses in CHO-AT(1) cells that were also inhibited upon repeated administrations. A smooth muscle cell line, which showed tachyphylaxis only to angiotensin II, became tachyphylactic also to [Lys2]angiotensin 11 after transfection with the angiotensin 11 AT(1) receptor gene. Our findings suggest that posttranscriptional control directed by the 3'- or the 5'-untranslated regions in the angiotensin 11 AT(1) receptor gene may play a role in modulating the signal transduction pathways involved in the mechanism of angiotensin 11 tachyphylaxis. (C) 2002 Elsevier Science B.V. All rights reserved.
Palavra-chave angiotensin II
[Lys(2)]angiotensin II
angiotensin II AT(1) receptor
recombinant
tachyphylaxis
CHO cell line
smooth muscle cell line
Idioma Inglês
Data de publicação 2002-03-29
Publicado em European Journal of Pharmacology. Amsterdam: Elsevier B.V., v. 439, n. 1-3, p. 13-19, 2002.
ISSN 0014-2999 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 13-19
Fonte http://dx.doi.org/10.1016/S0014-2999(01)01490-X
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000175230100002
Endereço permanente http://repositorio.unifesp.br/handle/11600/26801

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