Comparison of Fonsecaea pedrosoi sclerotic cells obtained in vivo and in vitro: ultrastructure and antigenicity

Comparison of Fonsecaea pedrosoi sclerotic cells obtained in vivo and in vitro: ultrastructure and antigenicity

Autor Silva, Jorge Pereira da Google Scholar
Alviano, Daniela Sales Google Scholar
Alviano, Celuta Sales Google Scholar
Souza, Wanderley de Google Scholar
Travassos, Luiz Rodolpho Autor UNIFESP Google Scholar
Diniz, José Antonio Picanço Google Scholar
Rozental, Sonia Google Scholar
Instituição Universidade Federal do Rio de Janeiro (UFRJ)
Fed Univ Para
Universidade Federal de São Paulo (UNIFESP)
Inst Evandro Chagas Fundacao Nacl Saude
Resumo The parasitic form of Fonsecaea pedrosoi from the hyperkeratotic layer of the skin was obtained from four patients with chromoblastomycosis. Primary cultures containing hyphae and conidia were successfully converted into sclerotic cells in the presence of 800 muM propranolol and low pH as described before [1]. the morphology of sclerotic cells of F pedrosoi obtained in vivo and in vitro was analyzed by light and electron microscopy. Their antigenicity was also compared by immunofluorescence microscopy and ELISA assays, using serum samples from untreated patients infected with F pedrosoi. Due to the similarity of the sclerotic cells obtained in vivo and in vitro, the latter can be more adequately in studies of host-parasite interactions in chromoblastomycosis. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
Palavra-chave chromoblastomycosis
antigenicity
sclerotic cell
ultrastructure
Fonsecaea pedrosoi
Idioma Inglês
Data de publicação 2002-03-25
Publicado em Fems Immunology and Medical Microbiology. Amsterdam: Elsevier B.V., v. 33, n. 1, p. 63-69, 2002.
ISSN 0928-8244 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 63-69
Fonte http://dx.doi.org/10.1111/j.1574-695X.2002.tb00574.x
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000175610300011
Endereço permanente http://repositorio.unifesp.br/handle/11600/26800

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