Differentiation and barrier formation of a cultured composite skin graft

Differentiation and barrier formation of a cultured composite skin graft

Autor Gragnani, A. Google Scholar
Morgan, JR Google Scholar
Ferreira, L. M. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo The differentiation and barrier formation of cultured composite skin grafts (CSGs) were assessed by histology and measurements of surface electrical capacitance (SEC) in vitro and in vivo. Keratinocytes cultured on the surface of acellular dermis were lifted to the air-liquid interface and analyzed for 30 days in vitro. Initially, SEC measurements of CSGs (n = 11) were high but quickly dropped between days 4 and 6 and remained steady for 30 days, indicating barrier formation by the epidermis. Histology of the CSGs (n = 6) demonstrated stratification of the epidermal cells and partial formation of the stratum corneum by day 3 that was complete by day 7. CSGs (n = 5) were transplanted to athymic mice, where they formed a stratified and differentiated epidermis. SEC measurements of CSGs remained low after transplant, suggesting that exposure to the air-liquid interface improved the maturation of CSGs in vitro prior to transplant.
Idioma Inglês
Data de publicação 2002-03-01
Publicado em Journal of Burn Care & Rehabilitation. Philadelphia: Lippincott Williams & Wilkins, v. 23, n. 2, p. 126-131, 2002.
ISSN 0273-8481 (Sherpa/Romeo, fator de impacto)
Publicador Lippincott Williams & Wilkins
Extensão 126-131
Fonte http://dx.doi.org/10.1097/00004630-200203000-00009
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000178765300007
Endereço permanente http://repositorio.unifesp.br/handle/11600/26795

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