Colocalization of heparin and histamine in the intracellular granules of test cells from the invertebrate Styela plicata (Chordata-Tunicata)

Colocalization of heparin and histamine in the intracellular granules of test cells from the invertebrate Styela plicata (Chordata-Tunicata)

Autor Cavalcante, MCM Google Scholar
Andrade, L. R. de Google Scholar
Santos-Pinto, C. D. Google Scholar
Straus, A. H. Google Scholar
Takahashi, H. K. Google Scholar
Allodi, S. Google Scholar
Pavao, MSG Google Scholar
Instituição Universidade Federal do Rio de Janeiro (UFRJ)
Universidade Federal de São Paulo (UNIFESP)
Resumo In most ascidian species the oocytes are surrounded by two types of accessory cells called follicle cells and test cells. Test cells are located on the periphery of oocytes and remain in the perivitelline space during egg development until hatching. Heparin and histamine were previously described in the test cells of the ascidian Styela plicata. in the present study, electron microscopy techniques were used to characterize the ultrastructure of the S. plicata test cells and to localize heparin and histamine in these cells. Test cells contain several intracellular granules with unique ultrastructural features. They are formed by elongated filaments composed of serial globules with an electron-lucent circle, containing a central electron-dense spot. Immunocytochemistry showed that heparin and histamine colocalize at the border of granule filaments in the test cell. Compound 48/80, a potent secretagogue of heparin-containing mast cells, also induced degranulation of test cells. According to these results, we suggest that test cells represent ancient effector cells of the innate immunity in primitive chordates. (C) 2002 Elsevier Science (USA). All rights reserved.
Palavra-chave ascidian test cells
heparin
histamine
immunocytochemistry
mast cells
Styela plicata
ultrastructure
Idioma Inglês
Data de publicação 2002-03-01
Publicado em Journal of Structural Biology. San Diego: Academic Press Inc Elsevier Science, v. 137, n. 3, p. 313-321, 2002.
ISSN 1047-8477 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 313-321
Fonte http://dx.doi.org/10.1016/S1047-8477(02)00007-2
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000176923000006
Endereço permanente http://repositorio.unifesp.br/handle/11600/26789

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