Trypanosoma cruzi: Isolation of an immunodominant peptide of TESA (trypomastigote excreted-secreted antigens) by gene cloning

Trypanosoma cruzi: Isolation of an immunodominant peptide of TESA (trypomastigote excreted-secreted antigens) by gene cloning

Autor Matsumoto, T. K. Google Scholar
Cotrim, P. C. Google Scholar
Silveira, J. F. da Google Scholar
Stolf, AMS Google Scholar
Umezawa, E. S. Google Scholar
Instituição Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Resumo Trypomastigote forms of Trypanosoma cruzi. excrete-secrete several molecules, which are immunodominant during the human infection. This complex antigenic mixture termed TESA (Trypomastigote Excreted-Secreted Antigens) presents a 150-160 kDa band that shows excellent specificity and sensitivity in Chagas' disease diagnosis by immunoblotting. Here we describe the isolation and the antigenic characterization of a recombinant peptide (TESA-1) containing a 10 kDa T. cruzi peptide that belongs to the 150-160 kDa TESA fraction. the clone was isolated by screening a T. cruzi genomic expression library with chagasic antibodies reactive to the 150-160 kDa band of TESA immunoblots. After expression, the recombinant peptide TESA-1 was purified and used to immunize rabbits, Anti-TESA-1 immunesera specifically recognized the 150-160 kDa fraction of TESA-blots from eight different T. cruzi strains. the TESA-1 peptide reacted with 82.2% of chagasic patient sera by immunoblotting, showing that it harbors most of the antigenic epitopes that account for the high reactivity of the 150-160 kDa band of TESA. (C) 20021 Elsevier Science Inc. All rights reserved.
Idioma Inglês
Data de publicação 2002-03-01
Publicado em Diagnostic Microbiology and Infectious Disease. New York: Elsevier B.V., v. 42, n. 3, p. 187-192, 2002.
ISSN 0732-8893 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 187-192
Fonte http://dx.doi.org/10.1016/S0732-8893(01)00348-0
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000174911300006
Endereço permanente http://repositorio.unifesp.br/handle/11600/26786

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