Avian anticoccidial activity of a novel membrane-interactive peptide selected from phage display libraries

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dc.contributor.author Silva, A. da
dc.contributor.author Kawazoe, U.
dc.contributor.author Freitas, FFT
dc.contributor.author Gatti, MSV
dc.contributor.author Dolder, H.
dc.contributor.author Schumacher, R. I.
dc.contributor.author Juliano, M. A.
dc.contributor.author Silva, M. J. da
dc.contributor.author Leite, A.
dc.date.accessioned 2016-01-24T12:33:17Z
dc.date.available 2016-01-24T12:33:17Z
dc.date.issued 2002-03-01
dc.identifier http://dx.doi.org/10.1016/S0166-6851(01)00439-X
dc.identifier.citation Molecular and Biochemical Parasitology. Amsterdam: Elsevier B.V., v. 120, n. 1, p. 53-60, 2002.
dc.identifier.issn 0166-6851
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/26783
dc.description.abstract In the present work. we describe the discovery of PW2, a novel peptide presenting in vitro activity against Eimeria acervulina and E. tenella sporozoites. PW2 as selected from phage display (Ph.D.) peptide libraries by an alternative method of panning using living purified E. acervulina sporozoites Lis targets. Our results showed that the peptide disrupts the sporozoite pellicle, resembling the effect caused by most natural antimicrobial peptides, PW2 peptide was also effective against fungi and showed low activity against Toxoplasma gondii tachyzoites, but no activity against Trypanosoma cruzi, Crithidia fasciculata epimastigotes, and bacteria. Additionally, the parasiticidal concentrations of PW2 produced a very low lytic effect on mammalian and avian cells. the effectiveness against Eimeria sporozoites and the absence of adverse effects to host cells indicates that PW2 may be used as a model to generate new drugs for the control of avian coccidiosis. (C) 2002 Elsevier Science B.V. All rights reserved. en
dc.format.extent 53-60
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Molecular and Biochemical Parasitology
dc.rights Acesso restrito
dc.subject anticoccidial peptides en
dc.subject Eimeria en
dc.subject phage display en
dc.title Avian anticoccidial activity of a novel membrane-interactive peptide selected from phage display libraries en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Estadual de Campinas (UNICAMP)
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, BR-13083970 Campinas, SP, Brazil
dc.description.affiliation Univ Estadual Campinas, Inst Biol, Dept Microbiol & Immunol, BR-13083970 Campinas, SP, Brazil
dc.description.affiliation Univ Estadual Campinas, Inst Biol, Dept Parasitol, BR-13083970 Campinas, SP, Brazil
dc.description.affiliation Univ Estadual Campinas, Inst Biol, Dept Biol Celular, BR-13083970 Campinas, SP, Brazil
dc.description.affiliation Univ São Paulo, Inst Quim, Dept Bioquim, BR-01498 São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Escola Paulista Med, Dept Biofis, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Escola Paulista Med, Dept Biofis, São Paulo, Brazil
dc.identifier.doi 10.1016/S0166-6851(01)00439-X
dc.description.source Web of Science
dc.identifier.wos WOS:000174312300005



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