Modulation of hyaluronan in the migratory pathway of mouse primordial germ cells

Modulation of hyaluronan in the migratory pathway of mouse primordial germ cells

Autor Soto-Suazo, M. Google Scholar
Abrahamsohn, P. A. Google Scholar
Pereda, J. Google Scholar
San Martin, S. Google Scholar
Nader, H. B. Google Scholar
Sampaio, L. O. Google Scholar
Zorn, TMT Google Scholar
Instituição Universidade de São Paulo (USP)
Univ Santiago Chile
Universidade Federal de São Paulo (UNIFESP)
Resumo In the present report we followed the distribution of hyaluronan during the phases of separation, migration, and colonization of the primordial germ cell migratory process. Hyaluronan was detected by the use of two cytochemical methods: (1) ruthenium hexammine trichloride (RHT) associated with enzymatic treatment with hyaluronate lyase and (2) a binding specific probe for hyaluronan. After RHT treatment the proteoglycans and/or glycosaminoglycans were observed as a mesh-work formed by electron-dense granules connected by thin filaments. After enzymatic digestion, no filaments could be detected in the migratory pathway. Quantitative analysis showed a close correlation between cell migration and the concentration of RHT-positive filaments. It was also shown that high amounts of hyaluronan were expressed in the separation phase and migration phases whereas during the colonization phase the amount of hyaluronan was clearly diminished. This study showed that the presence of primordial germ cells in each compartment of the migratory pathway was always accompanied by a high expression of hyaluronan. These results indicate that hyaluronan is an important molecule in the migratory process, providing the primordial germ cells with a hydrated environment that facilitates their movement toward the genital ridges.
Palavra-chave primordial germ cells
cell migration
extracellular matrix
Idioma Inglês
Data de publicação 2002-03-01
Publicado em Histochemistry and Cell Biology. New York: Springer, v. 117, n. 3, p. 265-273, 2002.
ISSN 0948-6143 (Sherpa/Romeo, fator de impacto)
Publicador Springer
Extensão 265-273
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000174866000009
Endereço permanente

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