Characterization of a Kunitz trypsin inhibitor with one disulfide bridge purified from Swartzia pickellii

Characterization of a Kunitz trypsin inhibitor with one disulfide bridge purified from Swartzia pickellii

Autor Cavalcanti, MDM Google Scholar
Oliva, MLV Google Scholar
Fritz, H. Google Scholar
Jochum, M. Google Scholar
Mentele, R. Google Scholar
Sampaio, M. Google Scholar
Coelho, LCBB Google Scholar
Batista, IFC Google Scholar
Sampaio, CAM Google Scholar
Instituição Universidade Federal de Pernambuco (UFPE)
Univ Pernambuco
Universidade Federal de São Paulo (UNIFESP)
Univ Munich
Resumo Swartzia pickellii is a Leguminosae that belongs to the Caesalpinioideae sub-family the Swartzia pickellii Trypsin Inhibitor (SWTI), a serine proteinase inhibitor was isolated from its seeds. SWTI is a single polypeptide chain protein and it's structure has 174 amino acid residues, it homologous to other Kunitz plant inhibitors, however shows some major differences: it contains only one disulfide bridge, instead two which are usually found in plant Kunitz inhibitors, and the SWTI reactive site does not contain the usual Arg or Lys residues at the putative reactive site (position 65). A glycosylation site was detected at Asn38 with 1188 kDa carbohydrate portion. the primary structure micro heterogeneity was found combining the sequence determination and mass spectrometry. Three forms of SWTI were actually defined: two glycosylated forms a 20,204 kDa (Arg 165) and 20,185 kDa (His 165) and one deglycosylated form 19,016 kDa (Arg 165), all of them contain a Met residue at position 130. (C) 2002 Elsevier Science (USA).
Assunto Swartzia pickellii
structure and specificity
blood coagulation
plant Kunitz inhibitor
trypsin inhibitor
human plasma kallikrein
primary structure determination
Idioma Inglês
Data 2002-03-01
Publicado em Biochemical and Biophysical Research Communications. San Diego: Academic Press Inc Elsevier Science, v. 291, n. 3, p. 635-639, 2002.
ISSN 0006-291X (Sherpa/Romeo, fator de impacto)
Editor Elsevier B.V.
Extensão 635-639
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000174202700031

Mostrar registro completo

Arquivos deste item

Arquivos Tamanho Formato Visualização

Não existem arquivos associados a este item.

Este item aparece na(s) seguinte(s) coleção(s)