Aberrant expression of growth differentiation factor-9 in oocytes of women with polycystic ovary syndrome

Aberrant expression of growth differentiation factor-9 in oocytes of women with polycystic ovary syndrome

Autor Teixeira Filho, Fabio Lopes Autor UNIFESP Google Scholar
Baracat, Edmund Chada Autor UNIFESP Google Scholar
Lee, Taek Hoo Google Scholar
Suh, Chang Suk Google Scholar
Matsui, Motozumi Google Scholar
Chang, R. Jeffrey Google Scholar
Shimasaki, Shunichi Google Scholar
Erickson, Gregory F. Google Scholar
Instituição Univ Calif San Diego
Universidade Federal de São Paulo (UNIFESP)
Kyungpook Natl Univ
Seoul Natl Univ
Resumo Polycystic ovary syndrome (PCOS) is a major cause of female infertility. Despite substantial effort, the etiology and pathogenesis of PCOS and polycystic ovaries (PCO) in women remain unknown. Recent studies in laboratory animals have documented a link between dysfunction of two oocyte growth factors, growth differentiation factor-9 (GDF-9) and bone morphogenetic factor-15 (BMP-15), and aberrant folliculogenesis. Because aberrant follicle development is a hallmark of PCOS, we wondered whether the expression patterns of these growth factors might be disrupted in PCOS and PCO oocytes. To address this issue, we compared the pattern and level of expression of GDF-9 and BMP-15 mRNA in ovaries from normal cycling (n = 12), PCOS (n = 5), and PCO (n = 7) patients. in situ hybridization studies showed that the expression of GDF-9 and BMP-15 is restricted to the oocytes in all ovaries examined. Interestingly, a decreased level of GDF-9 signal was observed in developing PCOS and PCO oocytes, compared with normal. This difference was evident throughout folliculogenesis, beginning at recruitment initiation and continuing through the small Graafian follicle stage. By contrast, there were no qualitative or quantitative changes in the expression of BMP-15 mRNA in PCOS oocytes during folliculogenesis. There were also no significant differences between normal and PCOS and PCOs in the levels of the mRNA encoding the housekeeping gene, cyclophilin. Together, these results indicate that the expression of GDF-9 mRNA is delayed and reduced in PCOS and PCO oocytes during their growth and differentiation phase. Because oocyte-derived GDF-9 is crucial for normal folliculogenesis and female fertility, we suggest that a dysregulation of oocyte GDF-9 expression may contribute to aberrant folliculogenesis in PCOS and PCO women.
Idioma Inglês
Data de publicação 2002-03-01
Publicado em Journal of Clinical Endocrinology & Metabolism. Chevy Chase: Endocrine Soc, v. 87, n. 3, p. 1337-1344, 2002.
ISSN 0021-972X (Sherpa/Romeo, fator de impacto)
Publicador Endocrine Soc
Extensão 1337-1344
Fonte http://dx.doi.org/10.1210/jcem.87.3.8316
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000174620600060
Endereço permanente http://repositorio.unifesp.br/handle/11600/26762

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