Transient resistance to B16F10 melanoma growth and metastasis in CD43-/- mice

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dc.contributor.author Fuzii, H. T.
dc.contributor.author Travassos, L. R.
dc.date.accessioned 2016-01-24T12:33:15Z
dc.date.available 2016-01-24T12:33:15Z
dc.date.issued 2002-02-01
dc.identifier http://dx.doi.org/10.1097/00008390-200202000-00003
dc.identifier.citation Melanoma Research. Philadelphia: Lippincott Williams & Wilkins, v. 12, n. 1, p. 9-16, 2002.
dc.identifier.issn 0960-8931
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/26754
dc.description.abstract CD43, the major transmembrane sialoglycoprotein of neutrophils, monocytes, T lymphocytes and platelets, is highly glycosylated and its high sialic acid content contributes to the strongly negative charge of cells. in this study the role of CD43 in melanoma development was addressed using CD43-/- mice (null mutated for the corresponding gene or knockout [KO]). Growth of B16F10 melanoma was retarded in the KO mice compared with the wild-type CD43+/+ control (WT). A marked difference in lung colonization and other metastatic foci was observed in the KO and WT mice up to 15 days after intravenous injection of tumour cells. the Initial resistance of KO mice was reversed with time, and in the long term there was no difference in the survival rate of the two animal groups. Transient resistance was attributed to increased adhesion of thrombin-activated platelets and leukocytes to melanoma and endothelial cells in KO mice. in the KO mice tumour emboli were found in the central portion of the lung more than at the lung periphery Immediately after intravenous Injection, in contrast to the WT mice. Activation of melanoma adhesion receptors by thrombin or TRAP stimulated lung colonization in WT but not KO mice. Therefore the correlation of tumour embolism and metastasis in short-term experiments depends on the nature and stability of interactions between the tumour and the blood/endothelial cells of the host. (C) 2002 Lippincott Williams Wilkins. en
dc.format.extent 9-16
dc.language.iso eng
dc.publisher Lippincott Williams & Wilkins
dc.relation.ispartof Melanoma Research
dc.rights Acesso restrito
dc.subject B16F10 tumour cells en
dc.subject CD43 en
dc.subject CD43-/-mice en
dc.subject cell adherence en
dc.subject melanoma en
dc.subject tumour emboli en
dc.title Transient resistance to B16F10 melanoma growth and metastasis in CD43-/- mice en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Universidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, Unidade Oncol Expt, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, Unidade Oncol Expt, BR-04023062 São Paulo, Brazil
dc.identifier.doi 10.1097/00008390-200202000-00003
dc.description.source Web of Science
dc.identifier.wos WOS:000174015400002



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