Role of kinins and sensory neurons in the rat pleural leukocyte migration induced by Phoneutria nigriventer spider venom

Role of kinins and sensory neurons in the rat pleural leukocyte migration induced by Phoneutria nigriventer spider venom

Autor Costa, SKP Google Scholar
Moreno, R. A. Google Scholar
Esquisatto, LCM Google Scholar
Juliano, L. Google Scholar
Brain, S. D. Google Scholar
De Nucci, G. Google Scholar
Antunes, E. Google Scholar
Instituição Universidade Estadual de Campinas (UNICAMP)
Universidade Federal de São Paulo (UNIFESP)
Kings Coll London
Resumo The leukocyte migration induced by Phoneutria nigriventer spider venom (PNV) has been investigated in rats using the pleurisy model. Intrapleural injection of PNV (10-100 mug/cavity) caused a dose- and time-dependent leukocyte accumulation. the bradykinin B-2 receptor antagonist Hoe 140 (0.5 mg/kg) substantially inhibited PNV-induced cell accumulation, whereas the angiotensin-converting enzyme inhibitor captopril (2 mg/kg) potentiated by 80% this effect. the non-specific kallikrein inhibitor aprotinin and the plasma kallikrein inhibitor soybean trypsin inhibitor greatly reduced PNV-induced leukocyte migration, whereas the selective tissue kallikrein inhibitor P-ac-F-S-R-EDDnp failed to affect PNV-induced responses. Treatment of rats with capsaicin (50 mg/kg) at the neonatal stage resulted in 67% inhibition of the PNV-induced cell migration. the neurokinin NK1 receptor antagonist SR140333, but not the NK2 receptor antagonist SR48968, reduced by 55% venom-induced cell accumulation. We conclude that bradykinin generation is involved in the PNV-induced pleural leukocyte migration in rats, where it can directly activate sensory nerves contributing to a neurogenic inflammatory mechanism. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
Palavra-chave Phoneutria nigriventer venom
kinins
sensory neurons
inflammation
pleurisy
capsaicin
Idioma Inglês
Data de publicação 2002-02-01
Publicado em Neuroscience Letters. Clare: Elsevier Sci Ireland Ltd, v. 318, n. 3, p. 158-162, 2002.
ISSN 0304-3940 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 158-162
Fonte http://dx.doi.org/10.1016/S0304-3940(01)02498-3
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000173643600012
Endereço permanente http://repositorio.unifesp.br/handle/11600/26750

Exibir registro completo




Arquivo

Arquivo Tamanho Formato Visualização

Não existem arquivos associados a este item.

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta