Subsites of trypsin active site favor catalysis or substrate binding

Subsites of trypsin active site favor catalysis or substrate binding

Autor Marana, SR Google Scholar
Lopes, A. R. Google Scholar
Juliano, L. Google Scholar
Juliano, M. A. Google Scholar
Ferreira, C. Google Scholar
Terra, W. R. Google Scholar
Instituição Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Resumo Enzymes enhance chemical reaction rates by lowering the activation energy, the energy barrier of the reaction leading to products. This occurs because enzymes bind the high-energy intermediate of the reaction (the transition state) more strongly than the substrate. We studied details of this process by determining the substrate binding energy (DeltaG(g), calculated from K-m values) and the activation energy (DeltaG(T), determined from k(cat)/K-m values) for the trypsin-catalyzed hydrolysis of oligopeptides. Plots of DeltaG(T) versus DeltaG(g) for oligopeptides with 15 amino acid replacements at each of the positions P-1', P-1, and P-2 were straight lines, as predicted by a derived equation that relates DeltaG(T) and DeltaG(g). the data led to the conclusion that the trypsin active site has subsites that bind moieties of substrate and of transition state in characteristic ratios, whichever substrate is used. This was unexpected and means that each subsite characteristically favors substrate binding or catalysis. (C) 2002 Elsevier Science.
Palavra-chave activation energy
binding energy
catalysis
Periplaneta americana
subsite role
substrate specificity
transition state
trypsin
Idioma Inglês
Data de publicação 2002-01-11
Publicado em Biochemical and Biophysical Research Communications. San Diego: Academic Press Inc Elsevier Science, v. 290, n. 1, p. 494-497, 2002.
ISSN 0006-291X (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 494-497
Fonte http://dx.doi.org/10.1006/bbrc.2001.6172
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000173480900075
Endereço permanente http://repositorio.unifesp.br/handle/11600/26731

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