Lipopolysaccharide-cell interaction and induced cellular activation in whole blood of septic patients

Lipopolysaccharide-cell interaction and induced cellular activation in whole blood of septic patients

Autor Salomão, Reinaldo Autor UNIFESP Google Scholar
Brunialti, MKC Google Scholar
Kallas, E. G. Google Scholar
Martins, P. S. Google Scholar
Rigato, O. Google Scholar
Freudenberg, M. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Max Planck Inst Immunbiol
Resumo We used biotinylated LPS (LPSb) and flow cytometry to study LPS-monocyte interaction and LPS-induced cellular activation in whole blood from septic patients (SP). Expression of surface activation markers was evaluated on monocytes (HLA-DR) and T lymphocytes (CD69 and CD95), and intracellular TNF-alpha on monocytes. Saturating curve and kinetics of LPSb detection on monocytes were similar in SP and healthy volunteers (HV). LPSb bound to monocytes was detected after 5 min of incubation in both groups, with a more pronounced decay in SP. Monocytes from SP had a lower expression of HLA-DR as compared to HV, both constitutive and upon LPS stimulation. the proportion of monocytes producing TNF-alpha after LPS stimulus was higher in HV than SP (mean+/-SD=25.2+/-14.2% and 2.2+/-2.6%, respectively, P<0.001). LPS-induced CD69 on T CD8(+) and CD8(-) lymphocytes was similar for patients and controls. Expression of CD95 on T lymphocytes was higher in SP as compared to HV on T CD8(+) cells (GMFI, mean&PLUSMN;SD=22.3&PLUSMN;14.6 and 8.6&PLUSMN;5.0, respectively, P=0.01) and CD8(-) cells (GMFI, mean&PLUSMN;SD=28.3&PLUSMN;7.7 and 14&PLUSMN;4.3 respectively, P<0.001). Thus, monocytes and lymphocytes seem to respond differently to LPS in septic patients. Monocyte hyporesponsiveness appears not to be related to a decreased binding capacity of LPS, but rather to an impaired signal transduction.
Idioma Inglês
Data de publicação 2002-01-01
Publicado em Journal of Endotoxin Research. Leeds: Maney Publishing, v. 8, n. 5, p. 371-379, 2002.
ISSN 0968-0519 (Sherpa/Romeo, fator de impacto)
Publicador Maney Publishing
Extensão 371-379
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000180150300009
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