Molecular, typing and antimicrobial susceptibility of vancomycin-resistant Enterococcus eaecium in Brazil

Molecular, typing and antimicrobial susceptibility of vancomycin-resistant Enterococcus eaecium in Brazil

Author Cereda, R. F. Google Scholar
Gales, A. C. Google Scholar
Silbert, S. Google Scholar
Jones, R. N. Google Scholar
Sader, H. S. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Iowa
Abstract OBJECTIVES: To characterize vancomycin-resistant enterococci (VRE) isolates and to evaluate the mode of dissemination of this pathogen in Brazil.DESIGN: We collected 22 vancomycin-resistant Enterococcus faecium isolates from 6 medical centers in São Paulo, Brazil, and 1 isolate from a medical center in Curitiba, Brazil.PARTICIPANTS: All Brazilian hospitals that had identified vancomycin-resistant E. faecium up to the beginning of this study gate 1999) contributed isolates to the study,METHODS: the isolates were susceptibility tested using the broth microdilution method and the E-test. the presence of vancomycin resistance genes (vanA vanB, vanC1, vanC2-3, and vanD) was evaluated by polymerase chain reaction; molecular typing was performed by pulsed-field gel electrophoresis (PFGE).RESULTS: the vanA gene was demonstrated in all vancomycin-resistant E. faecium, except for 1 isolate. None of the vancomycin resistance genes cited above was detected in the isolate from Curitiba, which was the first vancomycin-resistant E. faecium described in Brazil. An isolates were resistant to ampicillin and teicoplanin. the main clone remains susceptible to doxycycline and chloramphenicol, but intermediate to quinupristin-dalfopristin. PFGE analysis demonstrated 7 major PFGE patterns. A unique PFGE pattern with 4 subtypes was detected in 17 isolates from 4 different hospitals.CONCLUSION: the results of our study indicate the occurrence of intra- and interhospital dissemination of VRE in São Paulo, Brazil.
Language English
Date 2002-01-01
Published in Infection Control and Hospital Epidemiology. Thorofare: Slack Inc, v. 23, n. 1, p. 19-22, 2002.
ISSN 0899-823X (Sherpa/Romeo, impact factor)
Publisher Slack Inc
Extent 19-22
Origin http://dx.doi.org/10.1086/501962
Access rights Closed access
Type Article
Web of Science ID WOS:000173953000005
URI http://repositorio.unifesp.br/handle/11600/26719

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