Molecular, typing and antimicrobial susceptibility of vancomycin-resistant Enterococcus eaecium in Brazil

Molecular, typing and antimicrobial susceptibility of vancomycin-resistant Enterococcus eaecium in Brazil

Autor Cereda, R. F. Google Scholar
Gales, A. C. Google Scholar
Silbert, S. Google Scholar
Jones, R. N. Google Scholar
Sader, H. S. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Univ Iowa
Resumo OBJECTIVES: To characterize vancomycin-resistant enterococci (VRE) isolates and to evaluate the mode of dissemination of this pathogen in Brazil.DESIGN: We collected 22 vancomycin-resistant Enterococcus faecium isolates from 6 medical centers in São Paulo, Brazil, and 1 isolate from a medical center in Curitiba, Brazil.PARTICIPANTS: All Brazilian hospitals that had identified vancomycin-resistant E. faecium up to the beginning of this study gate 1999) contributed isolates to the study,METHODS: the isolates were susceptibility tested using the broth microdilution method and the E-test. the presence of vancomycin resistance genes (vanA vanB, vanC1, vanC2-3, and vanD) was evaluated by polymerase chain reaction; molecular typing was performed by pulsed-field gel electrophoresis (PFGE).RESULTS: the vanA gene was demonstrated in all vancomycin-resistant E. faecium, except for 1 isolate. None of the vancomycin resistance genes cited above was detected in the isolate from Curitiba, which was the first vancomycin-resistant E. faecium described in Brazil. An isolates were resistant to ampicillin and teicoplanin. the main clone remains susceptible to doxycycline and chloramphenicol, but intermediate to quinupristin-dalfopristin. PFGE analysis demonstrated 7 major PFGE patterns. A unique PFGE pattern with 4 subtypes was detected in 17 isolates from 4 different hospitals.CONCLUSION: the results of our study indicate the occurrence of intra- and interhospital dissemination of VRE in São Paulo, Brazil.
Idioma Inglês
Data de publicação 2002-01-01
Publicado em Infection Control and Hospital Epidemiology. Thorofare: Slack Inc, v. 23, n. 1, p. 19-22, 2002.
ISSN 0899-823X (Sherpa/Romeo, fator de impacto)
Publicador Slack Inc
Extensão 19-22
Fonte http://dx.doi.org/10.1086/501962
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000173953000005
Endereço permanente http://repositorio.unifesp.br/handle/11600/26719

Exibir registro completo




Arquivo

Arquivo Tamanho Formato Visualização

Não existem arquivos associados a este item.

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta