Macrophages take up triacylglycerol-rich emulsions at a faster rate upon co-incubation with native and modified LDL: An investigation on the role of natural chylomicrons in atherosclerosis

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dc.contributor.author Carvalho, Marcia DT
dc.contributor.author Harada, Lila M.
dc.contributor.author Gidlund, Magnus
dc.contributor.author Ketelhuth, Daniel FJ
dc.contributor.author Boschcov, Paulo [UNIFESP]
dc.contributor.author Quintão, Eder Carlos Rocha [UNIFESP]
dc.date.accessioned 2016-01-24T12:33:10Z
dc.date.available 2016-01-24T12:33:10Z
dc.date.issued 2002-01-01
dc.identifier http://dx.doi.org/10.1002/jcb.10020
dc.identifier.citation Journal of Cellular Biochemistry. New York: Wiley-liss, v. 84, n. 2, p. 309-323, 2002.
dc.identifier.issn 0730-2312
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/26692
dc.description.abstract Chylomicrons play a role in atherosclerosis, however, because the mechanisms involved in the cell uptake of these particles are not fully understood, investigations were carried out using a radioactively labeled protein-free triacylglycerol-rich emulsion incubated with peritoneal macrophages obtained from normal and apoE-knockout mice. Experiments were done in the presence of substances that inhibit several endocytic processes: EDTA for low density lipoprotein receptor, fucoidan for scavenger receptor, cytochalasin B for phagocytosis, and a lipopolysaccharide for lipoprotein lipase. in addition, triacylglycerol-rich emulsions were also prepared in the presence of native or modified radioactively labeled low density lipoprotein particles that are known to accumulate in the arterial intima. Probucol was also used to prevent the possible role played by an antioxidant in triacylglycerol-rich emulsion uptake. We have shown that triacylglycerol-rich emulsion alone is taken up by a coated-pit-dependent mechanism, mediated by macrophage secretion of apolipoprotein E. Furthermore, native, aggregated, acetylated, and moderately macrophage-oxidized low density lipoprotein stimulate the uptake of a triacylglycerol-rich emulsion through several mechanisms such as an actin-dependent pathway, scavenger receptors, and lipolysis mediated by lipoprotein lipase. On the other hand, in spite of the interaction of low density lipoprotein forms with a triacylglycerol-rich emulsion, the cellular triacylglycerol-rich emulsion uptake is impaired by copper-oxidized low density lipoprotein, possibly due to its diminished affinity towards lipoprotein lipase. We have also shown that macrophages take up aggregated low density lipoprotein better than the acetylated or oxidized forms of low density lipoprotein. (C) 2001 Wiley-Liss, Inc. en
dc.format.extent 309-323
dc.language.iso eng
dc.publisher Wiley-Blackwell
dc.relation.ispartof Journal of Cellular Biochemistry
dc.rights Acesso restrito
dc.subject triacylglycerol-rich emulsions en
dc.subject ethylenediaminetetraacetic acid en
dc.subject low density lipoprotein en
dc.subject fucoidan en
dc.subject cytochalasin B en
dc.subject lipopolysaccharide en
dc.title Macrophages take up triacylglycerol-rich emulsions at a faster rate upon co-incubation with native and modified LDL: An investigation on the role of natural chylomicrons in atherosclerosis en
dc.type Artigo
dc.rights.license http://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ São Paulo, Sch Med, Lipids Lab, BR-01246903 São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Inst Biomed Sci, Dept Immunol, São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Biophys, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Biophys, São Paulo, Brazil
dc.identifier.doi 10.1002/jcb.10020
dc.description.source Web of Science
dc.identifier.wos WOS:000172911000011



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