Adenoviral-mediated gene transfer to the filtering bleb in rabbits

Adenoviral-mediated gene transfer to the filtering bleb in rabbits

Autor Skaf, Mirian Autor UNIFESP Google Scholar
di Martino, D. S. Google Scholar
Mello, Paulo Augusto de Arruda Autor UNIFESP Google Scholar
Varma, R. Google Scholar
Instituição Doheny Eye Inst
Univ So Calif
Universidade Federal de São Paulo (UNIFESP)
Resumo Purpose: To determine if genes can be transferred to fibroblasts in the filtering bleb using adenoviral vectors. Materials and Methods: Twelve New Zealand albino rabbits underwent bilateral full-thickness sclerostomies. On postoperative day I an adenoviral vector carrying a reporter gene (lacZ) was injected into the right-eye bleb and saline was injected into the left eye bleb of each rabbit. Three rabbits were euthanized on each of the after days (days 3, 7, 14, and 21). the eyes were enucleated and tissue samples from the filtering bleb were processed for beta -galactosidase activity (a marker for lacZ gene expression) and expression of vimentin (a fibroblast marker). the median number of cells per high-power field with both beta -galactosidase activity and vimentin expression on days 3, 7, 14, and 21 in the right and left eyes were counted to determine adenoviral-mediated gene expression in fibroblasts. Results: in the adenoviral vector-treated eyes, the median number of cells per high-power field with both beta -galactosidase and vimentin expression on days 3, 7, 14, and 21 was 83, 100, 1, and 0, respectively. No beta -galactosidase activity was noted in the saline-treated eyes. Conclusions: Adenoviral vectors can transfer genes to fibroblasts in filtering blebs after glaucoma surgery. the peak efficiency of gene transfer to fibroblasts occurred 7 days after glaucoma surgery. These studies show a potential for genetic manipulation of fibroblast activity in filtering blebs after glaucoma surgery.
Assunto gene expression
glaucoma surgery
wound healing
Idioma Inglês
Data 2001-12-01
Publicado em Journal of Glaucoma. Philadelphia: Lippincott Williams & Wilkins, v. 10, n. 6, p. 470-476, 2001.
ISSN 1057-0829 (Sherpa/Romeo, fator de impacto)
Editor Lippincott Williams & Wilkins
Extensão 470-476
Fonte http://dx.doi.org/10.1097/00061198-200112000-00006
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000172730900006
URI http://repositorio.unifesp.br/handle/11600/26668

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