Comparison of the immunogenic properties of recombinant proteins representing the Plasmodium vivax vaccine candidate MSP1(19) expressed in distinct bacterial vectors

Comparison of the immunogenic properties of recombinant proteins representing the Plasmodium vivax vaccine candidate MSP1(19) expressed in distinct bacterial vectors

Autor Cunha, M. G. Google Scholar
Rodrigues, M. M. Google Scholar
Soares, I. S. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Fed Univ Para
Universidade de São Paulo (USP)
Resumo The 19 kDa C-terminal region of the merozoite surface protein 1 (MSP1(19)) is one of the most promising vaccine candidates against the erythrocytic forms of malaria. in the present study, we used three different Escherichia coli expression vectors to generate five recombinant proteins representing the MSP1(19) of Plasmodium vivax. These proteins were compared for reactivity with a panel of sera from individuals naturally exposed to P. vivax and for their immunogenicity in mice. Among the proteins studied, MSPI 19 expressed by the vector pET (His(6)-MSP1(19)) was better recognized by the antibodies of several individuals exposed to P. vivax. the addition of the T-cell Pan-allelic DR epitope (PADRE) did not alter the recognition of this recombinant protein by human antibodies. Although recombinant proteins were immunogenic to mice, immunization with MSP1(19) expressed by the pET or pGEX vectors induced significantly higher antibody titers than a protein produced by the pMAL vector. the antibody immune response elicited by His(6)-MSP1(19) containing the PADRE epitope was compared using different adjuvant formulations. After only two immunizing doses, antibody titers induced in the presence of the adjuvants TiterMax, MPL/TDM/CWS or alum plus CpG ODN 1826 were as high as Liters generated by complete Freund's adjuvant. We concluded that, among the bacterial recombinant proteins, MSPI1(19) expressed by the vector pET should be selected for further evaluation in pre-clinical immunizations against P. vivax. (C) 2001 Published by Elsevier B.V.
Palavra-chave malaria
recombinant proteins
P. vivax
Idioma Inglês
Data de publicação 2001-11-12
Publicado em Vaccine. Oxford: Elsevier B.V., v. 20, n. 3-4, p. 385-396, 2001.
ISSN 0264-410X (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 385-396
Fonte http://dx.doi.org/10.1016/S0264-410X(01)00359-0
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000172153400014
Endereço permanente http://repositorio.unifesp.br/handle/11600/26665

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